Core screening dimensions and standardized use points of fetal bovine serum for cell culture

The central role of fetal bovine serum in the cell culture system

Fetal bovine serum is a widely used natural culture medium supplementary ingredient in the field of cell culture. It contains nutrients required for the proliferation and maintenance of phenotypes of cells such as growth factors, adherent cofactors, hormones, trace elements, and transporters. It can provide nutritional support for cells cultured in vitro that approximates the physiological environment in vivo. Currently, it is widely used in many scenarios such as monoclonal antibody research and development, cell therapy technology development, viral vector preparation, and basic life science research.Due to the difference between the raw material source and the preparation process, there are certain differences in the natural composition of different batches of fetal bovine serum, which will directly affect the cell growth state and the repeatability of the experimental results.

Core Quality Screening Dimension of Fetal Bovine Serum

R&D and production personnel can combine their own cell types and application scenarios to screen suitable fetal bovine serum products from the following dimensions:

Traceability qualification and origin information: Priority is given to products with complete supply chain traceability documents. Non-epidemic area certificates and official veterinary health certificates in blood sampling areas can be verified, which meet the general traceability requirements for raw materials for biomedical research and development to avoid unknown risks from unknown sources of serum.

Coverage of exogenous factor detection: Common detection items include bovine viral diarrhea virus, bovine parvovirus, mycoplasma, chlamydia, etc. Different application scenarios can select products corresponding to the detection level according to the sensitivity of cells and the research and development stage. Scenarios with special prevention and control needs can also select categories treated with γ-ray irradiation to reduce active virus pollution.