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Product data sheet PD-15211ES
BsiWI (10 U/μL) BsiWI (10 U/μL)
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BsiWI (10 U/μL)

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载体构建
Product no.15211ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15211ES75 现货 $618.00 促$655.00
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Cat.No Spec Price Status
15211ES75 300 U $618.00促$655.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15211ES
Specification识别位置5'-C↓GTACG-3'3'-GCATG↑C-5'酶活10 U/μL反应条件1×BsiWI Buffer;55℃孵育失活条件80℃温育20 min酶活定义单位活性单位(U)是指在 50 μL反应体系中,55℃ 1 h内可以完全酶切
Origin翌圣生物科技(上海)股份有限公司
应用方法 载体构建用于DNA片段克隆和载体构建
生物特性参数 试剂类型限制性内切酶 应用载体构建
技术参数 浓度10 U/μL 缓冲液组分1×BsiWI Buffer,含重组白蛋白 储存条件-25~-15℃保存,有效期2年 识别序列5'-C↓GTACG-3' 3'-GCATG↑C-5' 反应条件1×BsiWI Buffer;55℃孵育 失活条件80℃温育20 min 酶活定义在50 μL反应体系中,55℃ 1 h内完全酶切1 µg p615所需的酶量 同裂酶PspLI Pfl23II 甲基化修饰影响CpG:剪切受阻;Dam、Dcm、EcoKI、EcoBI:无影响
实验类型 限制性酶切识别并切割特定DNA序列

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