Proteinase K Powder 蛋白酶K冻干粉（RNase-Free, DNase-Free）

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分子生物学核酸提取与纯化

Product no.10401ES Sizes5 options Documents1 files SourceYeasen · Official

Cat. No. 10401ES60

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10401ES60 100 mg $168.00促~~$252.00~~ 现货 Enquire

10401ES80 1 g $1,318.00促~~$2,252.00~~ 现货 Enquire

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10401ES90 5 g $9,475.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No10401ES

Specification源于基因重组酵母菌株。不含 RNase 和 DNase。在尿素和 SDS 中活性稳定存在。在较广的 pH 范围内（pH 4.0 – 12.0）均有活性，最佳 pH 范围为 pH 7.5 - 9.0。

Origin翌圣生物科技(上海)股份有限公司

应用方法 分子生物学通用工具酶 核酸提取与纯化去除核酸酶

生物特性参数 试剂类型蛋白酶 应用制备脉冲电泳的染色体DNA；蛋白质印迹；去除DNA和RNA制备中的核酸酶

技术参数 分子量约29kDa 活性pH范围pH 4.0-12.0，最佳pH 7.5-9.0 稳定性在尿素和SDS中活性稳定 常用工作浓度50-100µg/mL 产品形式冻干粉 来源基因重组酵母菌株 无核酸酶不含RNase和DNase 储存条件2~8℃干燥保存，有效期3年；更长期保存可置于-25~-15℃冷冻保存

实验类型 脉冲电泳制备染色体DNA 蛋白质印迹蛋白质检测

FAQ

Q：蛋白酶 K 主要有哪些应用？

A：原位杂交样本制备；核酸纯化等实验蛋白质的切割清除。

Documents & Citations

蛋白纯化产品目录 Open document

Published citations

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[2] Zhang Z, Mou Z, Xu C, et al. Autophagy-associated circular RNA hsa_circ_0007813 modulates human bladder cancer progression via hsa-miR-361-3p/IGF2R regulation. Cell Death Dis. 2021;12(8):778. Published 2021 Aug 7. doi:10.1038/s41419-021-04053-4(IF:8.469)

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[5] Ma J, Chen S, Hao L, et al. Hypermethylation-mediated down-regulation of lncRNA TBX5-AS1:2 in Tetralogy of Fallot inhibits cell proliferation by reducing TBX5 expression. J Cell Mol Med. 2020;24(11):6472-6484. doi:10.1111/jcmm.15298(IF:4.486)

[6] Shi L, Gong Y, Zhuo L, Wang S, Chen S, Ke B. Methyltransferase-like 3 upregulation is involved in the chemoresistance of non-small cell lung cancer. Ann Transl Med. 2022;10(3):139. doi:10.21037/atm-21-6608(IF:3.932)

[7] Ma J, Chen S, Hao L, et al. Long non-coding RNA SAP30-2:1 is downregulated in congenital heart disease and regulates cell proliferation by targeting HAND2. Front Med. 2021;15(1):91-100. doi:10.1007/s11684-020-0778-5(IF:3.297)

[8] Huang Q, Xie Y, Yang Z, et al. The cytoplasmic loops of AgrC contribute to the quorum-sensing activity of Staphylococcus aureus. J Microbiol. 2021;59(1):92-100. doi:10.1007/s12275-021-0274-x(IF:2.845)

[9] Guo T, Xing Y, Chen Z, et al. Core-binding factor beta is required for osteoblast differentiation during fibula fracture healing. J Orthop Surg Res. 2021;16(1):313. Published 2021 May 14. doi:10.1186/s13018-021-02410-9(IF:2.359)

[10] Zhang W, Zhang T, Yan Y, et al. Exosomal miR-22-3p Derived from Chronic Rhinosinusitis with Nasal Polyps Regulates Vascular Permeability by Targeting VE-Cadherin. Biomed Res Int. 2020;2020:1237678. Published 2020 Nov 12. doi:10.1155/2020/1237678(IF:2.276)

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