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Product data sheet PD-40601ES

GMyc-PCR Mycoplasma Test Kit GMyc-PCR支原体检测试剂盒

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In stock Cell Biology Reagents 翌圣生物 Yeasen

GMyc-PCR Mycoplasma Test Kit GMyc-PCR支原体检测试剂盒

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PCR支原体污染检测细胞培养质控

Product no.40601ES Sizes2 options Documents2 files SourceYeasen · Official

Cat. No. 40601ES10

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40601ES10 10 assays ¥308.00促~~¥327.00~~ 现货 Enquire

40601ES20 20 assays ¥528.00促~~¥557.00~~ 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No40601ES

Specification1、灵敏度高：更低含量的支原体也能检测到，能够更早发现支原体污染2、特异性强：和其他近亲缘菌样本同时检测，仅支原体被检出3、适用范围广：可以用直接用细胞培养上清液检测

Origin翌圣生物科技(上海)股份有限公司

技术平台 PCR

应用方法 支原体污染检测检测细胞培养物等生物材料中支原体感染 细胞培养质控定期监测并预防支原体污染

适用物种种属 支原体 口腔支原体 精氨酸支原体 猪鼻支原体 发酵支原体 人型支原体 唾液支原体 肺支原体 梨支原体

检测方法 PCR法 琼脂糖凝胶电泳

生物特性参数 检测靶标支原体DNA 试剂类型PCR支原体检测试剂盒 检测类型PCR法 交叉反应性与所列近缘细菌无交叉，仅支原体被检出 应用细胞培养物、实验动物分泌物、动物血清等支原体检测

样本类型 细胞培养上清液 细胞培养物 动物血清 实验动物分泌物 生物材料

技术参数 检测限10 copies/μL 灵敏度可检出更低含量支原体，更早发现污染 特异性仅特异性扩增支原体DNA，与近缘菌无交叉 引物靶标支原体16S-23S rRNA保守区域 检测时间PCR扩增及电泳分析只需数小时 储存条件-25~-15℃避光保存 运输条件干冰运输 有效期18个月 货号40601ES

实验类型 PCR扩增支原体16S-23S rRNA保守区 琼脂糖凝胶电泳分析PCR扩增产物

FAQ

Q：检测准吗？巢式 pcr 什么意思？为什么要做两轮 pcr？

A：巢式 PCR 通过两轮扩增的方式，增加了产品的特异性和灵敏度，可以对 10 拷贝的支原体进行检测。

Q：GMyc-PCR 支原体检测试剂盒，能测细胞吗？细胞支原体测定方法是什么样的？

A：可以的，如果使用细胞悬液做样品，则需要提取DNA 后再进行 PCR。加入量一般为反应体系 1/10 的量或更少。

Q：对于同一个样品，为什么一步法恒温试剂盒检测结果为阳性，而 PCR 检测为阴性呢？

A：一步法恒温检测试剂盒检测的支原体的种类是 27 种，而 PCR 检测方法检测的是常见的 13 种支原体。样品中污染的支原体可能不包括在 PCR 法检测的支原体种类内。

Q：巢式 PCR 法和一步法恒温支原体检测试剂盒他们的优势在哪些方面，怎么选择？

A：PCR 法的灵敏度高于一步法检测，而一步法的灵敏度是 10^3 个拷贝才能检测出来。一步法检测的细胞支原体种类达 20 多种多于 PCR 法。

Q：GMyc-PCR 支原体检测结果如何判断？

A：GMyc-PCR 支原体检测方法通过巢式 PCR 的方式获得检测结果。整个实验由 2 轮PCR 组成，检测结果以第二轮为准。

Q：说明书中写的是不加loading buffer，直接点样就可以，我点样之后所哟有样品均没有条带（包含阳性对照），阳性对照加了loading buffeer才有条带。

A：经确定客户配胶的时候未加入EB染料，他们的染料是加在loading buffer中的，所以不加入自己的loading buffer跑不出条带。

EB加入方式：

1.如果配琼脂糖凝胶的时候加入EB染料，跑胶的的样品中不需要加入了;

2.如果配琼脂糖凝胶的时候未加入EB染料，跑胶的的样品中需要加入EB染料。

Q：阳性对照是哪种支原体呢？

A：猪鼻子支原体。

Q: 第二轮扩增的模板用什么稀释?

A: 可以用双蒸水稀释。

Documents & Citations

支原体qPCR法检测整体解决方案 Open document 支原体污染整体解决方案(检测去除预防) Open document

Published citations

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