Recombinant Human IGFBP-3 Protein,His Tag

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HEK293表达系统抗体药物靶点研究免疫检测

Product no.95923ES Sizes3 options SourceYeasen · Official

Cat. No. 95923ES25

现货 $1,065.00

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Cat.No Spec Price Status

95923ES25 25 μg $1,065.00 现货 Enquire

95923ES60 100 μg $3,495.00 现货 Enquire

95923ES76 500 μg $12,235.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No95923ES

Specification25 μg

Storage-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。

Shelf Life1年

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 抗体药物靶点研究作为靶点蛋白用于抗体药物筛选与验证 免疫检测可作为标准品或捕获蛋白用于ELISA、SPR等分析

适用物种种属 Human

检测方法 酶联免疫吸附实验 表面等离子体共振 SDS-PAGE 鲎试剂法

生物特性参数 蛋白类型重组蛋白/靶点蛋白/抗体药物靶点蛋白 种属来源Human 生物活性描述Immobilized Human IGFBP-3 His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IGF-II hFc Tag with the EC50 of 0.19μg/ml determined by ELISA. Human IGFBP-3 His Tag captured on CM5 Chip via anti-his antibody can bind Human IGF-I hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay. 应用作为抗体药物靶点蛋白，用于免疫检测、药物筛选及相关研究

技术参数纯度> 95% as determined by SDS-PAGE 内毒素水平< 1.0 EU per μg by the LAL method 分子量Predicted MW 29.8 kDa; migrates to 45-60 kDa due to glycosylation 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization 储存条件-25 ~ -15℃保存，有效期1年；复溶后，-85 ~ -65℃保存，3个月有效期 活性EC50=0.19 μg/ml (ELISA); KD=2.32 nM (SPR) 表达序列Gly28-Lys291 表达系统HEK293 标签C-His 外观Lyophilized Powder 复溶方法开盖前先离心，使用蒸馏水复溶，复溶浓度超过100 μg/mL，建议分装避免反复冻融 蛋白登录号AAH18962