品牌翌圣生物 Yeasen
货号 / 型号95209ES
纯度> 95% as determined by SDS-PAGE and HPLC.
规格25 μg
性状Lyophilized Powder
储存条件-25 ~ -15℃保存,收到货之后有效期1年。
复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293表达系统
应用方法
抗体药物靶点研究药物研发
适用物种种属
人
检测方法
LAL法
SDS-PAGE
HPLC
ELISA
生物特性参数
蛋白类型重组蛋白/靶点蛋白
种属来源Human (人)
生物活性描述Immobilized Human IL-1R2
No Tag at 2μg/ml (100μl/well) on plate; dose response curve for Biotinylated Human IL-1 Beta
His Tag with EC50 of 0.21μg/ml determined by ELISA.
应用抗体药物靶点蛋白研究
样本类型
重组蛋白
技术参数
纯度> 95% as determined by SDS-PAGE and HPLC
内毒素水平< 1.0 EU per μg by LAL method
分子量Predicted MW 37.78 kDa; migrates to 50-70 kDa by SDS-PAGE due to glycosylation
活性ELISA: EC50 of 0.21 μg/ml for Biotinylated Human IL-1 Beta
His Tag
制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4); normally 8% trehalose added as protectant before lyophilization
储存条件-25 ~ -15°C
valid for 1 year; after reconstitution under sterile conditions
-85 ~ -65°C
valid for 3 months
表达系统HEK293
标签No Tag
表达序列Phe14-Glu343
外观Lyophilized Powder
复溶方法Centrifuge before opening; reconstitute lyophilized protein with distilled water to >100 μg/mL; aliquot for single-use to avoid repeated freeze-thaw
Accession号NP_004624.1
产品别称IL-1RT2
IL-1R-beta
IL-1RT-2
IL-1R-2
实验类型
ELISA生物活性检测
重组蛋白表达目的蛋白生产