品牌翌圣生物 Yeasen
货号 / 型号94722ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293细胞表达系统
适用物种种属
小鼠
检测方法
表面等离子共振(SPR)
SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)
鲎试剂法(LAL)
Tris-Bis聚丙烯酰胺凝胶电泳(Tris-Bis PAGE)
生物特性参数
蛋白类型重组蛋白
蛋白名称Galectin-3 (半乳糖凝集素-3)
种属来源小鼠
生物活性描述Mouse Galectin-3
His Tag immobilized on CM5 Chip can bind Mouse LGALS3BP
His Tag with an affinity constant of 17.56 nM as determined in SPR assay.
产品别称AGE-R3
Gal-3
GAL3
GALBPCBP35
技术参数
纯度> 95% as determined by SDS-PAGE.
内毒素水平< 1.0 EU per μg by the LAL method.
分子量The protein has a predicted MW of 28.5 kDa. Due to glycosylation
the protein migrates to 43-50 kDa based on Tris-Bis PAGE result.
制剂形式Lyophilized from 0.22μm filtered solution in PBS
300mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
活性Mouse Galectin-3
His Tag immobilized on CM5 Chip can bind Mouse LGALS3BP
His Tag with an affinity constant of 17.56 nM as determined in SPR assay.
储存条件-25 ~ -15℃保存,收到货之后有效期1年。复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期。
表达系统HEK293
标签C-His
外观Lyophilized Powder
复溶方法开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
表达序列Ala2-Ile264
Accession编号P16110
实验类型
SPR检测生物活性
SDS-PAGE检测纯度
LAL检测内毒素水平
Tris-Bis PAGE检测分子量