品牌翌圣生物 Yeasen
货号 / 型号94641ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293细胞表达系统
应用方法
抗体药物靶点蛋白作为重组靶点蛋白用于抗体药物相关研究
适用物种种属
食蟹猴
检测方法
ELISA
SDS-PAGE
HPLC
LAL
生物特性参数
蛋白类型重组蛋白
种属来源Cynomolgus(食蟹猴)
生物活性描述Immobilized Cynomolgus MMP-9
His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody
hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
产品别称CLG4B
GELB
MMP-9
Gelatinase B
AccessionA0A2K5UU71
技术参数
纯度> 95% as determined by SDS-PAGE and HPLC.
内毒素水平< 1.0 EU per μg by the LAL method.
分子量The protein has a predicted MW of 77.44 kDa. Due to glycosylation
the protein migrates to 85-100 kDa based on SDS-PAGE result.
表达序列Ala20-Asp706
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
活性Immobilized Cynomolgus MMP-9
His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody
hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
表达系统HEK293
标签C-His
外观Lyophilized Powder
复溶方法开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
储存条件-25 ~ -15℃保存,收到货之后有效期1年。复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期。
实验类型
ELISA检测抗体结合活性
SDS-PAGE纯度检测
HPLC纯度检测
LAL内毒素检测