Recombinant Human RANKL/TNFSF11/CD254 Protein

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HEK293表达系统骨代谢研究免疫系统研究乳腺发育研究中枢神经系统体温调节研究

Product no.94496ES Sizes3 options SourceYeasen · Official

Cat. No. 94496ES60

现货 ¥5,295.00

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94496ES60 100 μg ¥5,295.00 现货 Enquire

94496ES76 500 μg ¥17,685.00 现货 Enquire

94496ES25 25 μg ¥1,455.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No94496ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 骨代谢研究研究靶点 免疫系统研究研究靶点 乳腺发育研究研究靶点 中枢神经系统体温调节研究研究靶点

适用物种种属人

检测方法 SDS-PAGE SEC-HPLC ELISA SPR

生物特性参数 蛋白类型重组蛋白 种属来源Human (人) 生物活性描述Immobilized Human RANKL No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TNFRSF11A hFc Tag with the EC50 of 13.6ng/ml determined by ELISA. Also Human TNFRSF11B His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL No Tag with an affinity constant of 0.030 nM as determined in SPR assay. 应用骨代谢研究；免疫系统研究；乳腺发育研究；中枢神经系统体温调节研究

技术参数纯度> 90% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Predicted MW 20.5 kDa; migrates to 30-60 kDa by SDS-PAGE due to glycosylation. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 活性EC50=13.6 ng/ml (ELISA); KD=0.030 nM (SPR) 表达序列Gly63-Asp244 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 AccessionO14788-2 表达系统HEK293 标签No Tag 外观Lyophilized Powder