品牌翌圣生物 Yeasen
货号 / 型号94300ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293表达系统
应用方法
抗体药物靶点研究靶点蛋白应用
适用物种种属
人
检测方法
SDS-PAGE
HPLC
ELISA
SPR
生物特性参数
蛋白类型重组蛋白
种属来源Human
生物活性描述ELISA: Immobilized Human IL-15RA&IL-15
His Tag at 2μg/ml (100μl/well) on the plate. Dose 21.5ng/ml determined by ELISA. SPR: Human IL-2 R beta&IL-2 R gamma
hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2
No Tag with an affinity constant of 0.114 nM; bind Human IL-15Ra&IL-15
His Tag with an affinity constant of 0.084 nM.
应用抗体药物靶点研究
样本类型
重组蛋白
技术参数
表达系统HEK293
标签C-hFc
纯度>95% as determined by SDS-PAGE and HPLC
内毒素水平<1.0 EU per μg by the LAL method
分子量Predicted MW: 51.3 kDa (IL-2 R beta)&54.6 kDa (IL-2 R gamma); Observed: 65-70 kDa&80-100 kDa (Tris-Bis PAGE)
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
外观Lyophilized Powder
复溶方法Centrifuge before opening. Reconstitute lyophilized protein in distilled water to a concentration over 100 μg/mL. Recommend aliquot to avoid repeated freeze-thaw cycles.
储存条件-25 ~ -15℃保存,收到货之后有效期1年。复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期。
表达序列Ala27-Thr240 (IL-2 R beta)&Leu23-Ala262 (IL-2 R gamma)
AccessionP14784(IL-2 R beta)&P31785-1(IL-2 R gamma)
实验类型
ELISA测定结合活性
SPR测定亲和力