品牌翌圣生物 Yeasen
货号 / 型号94126ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
技术平台
大肠杆菌表达系统
应用方法
抗体药物靶点研究作为靶点蛋白
适用物种种属
人
检测方法
LAL法
SDS-PAGE
ELISA
生物特性参数
蛋白类型重组蛋白
种属来源人(Human)
生物活性描述【ELISA Data】Immobilized Human CCL5
His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CXCL4
His Tag with the EC50 of 1.1μg/ml determined by ELISA.
应用抗体药物靶点研究
技术参数
纯度> 95% as determined by SDS-PAGE.
内毒素水平< 1.0 EU per μg by the LAL method.
分子量The protein has a predicted MW of 10.68 kDa. The protein migrates to 13 kDa based on Tris-Bis PAGE result.
制剂形式Lyophilized from 0.22μm filtered solution in 4mM HCl (pH 3.0). Normally 8% trehalose is added as protectant before lyophilization.
活性EC50=1.1μg/ml (by ELISA)
储存条件-25 ~ -15℃保存,收到货之后有效期1年。复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期。
表达序列Glu32-Ser101
表达系统E.coli
标签N-His-Avi
Accession号NP_002610.1
复溶方法Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCl (pH 3.0).
外观Lyophilized Powder
实验类型
ELISA检测生物活性
SDS-PAGE检测纯度
LAL法检测内毒素水平