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Product data sheet PD-40302ES
Annexin V-FITC/PI Apoptosis Detection Kit  Annexin V-FITC/PI 细胞凋亡检测试剂盒 Annexin V-FITC/PI Apoptosis Detection Kit  Annexin V-FITC/PI 细胞凋亡检测试剂盒
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Annexin V-FITC/PI Apoptosis Detection Kit Annexin V-FITC/PI 细胞凋亡检测试剂盒

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流式细胞仪荧光显微镜细胞凋亡研究
Product no.40302ES Sizes5 options Documents1 files SourceYeasen · Official
Cat. No. 40302ES20
现货 $368.00 促$480.00
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Specifications & Enquiry

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Cat.No Spec Price Status
40302ES20 20 T $368.00促$480.00 现货 Enquire
40302ES50 50 T $688.00促$960.00 现货 Enquire
40302ES60 100 T $988.00促$1,680.00 现货 Enquire
40302ES08 5 T $120.00 现货 Enquire
40302ES00 5 T Ask 咨询 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No40302ES
Specification检测便捷、准确
Origin翌圣生物科技(上海)股份有限公司
技术平台 流式细胞仪 荧光显微镜
应用方法 细胞凋亡研究用于流式细胞仪或荧光显微镜定量检测细胞凋亡
检测方法 流式细胞术 荧光显微镜检测
生物特性参数 应用细胞凋亡检测
样本类型 细胞
技术参数 储存条件-20℃避光,避免反复冻融,一年有效;4℃避光半年有效 运输条件冰袋(wet ice)运输 Annexin V分子量35-36 kDa
实验类型 Annexin V-FITC/PI细胞凋亡检测检测细胞早期凋亡及晚期凋亡/坏死

FAQ

Q:Annexin V 和 JC-1、Tunel 细胞凋亡检测的区别?

A: Annexin V 是检测细胞早期凋亡的试剂,JC-1 是检测细胞中期凋亡的试剂、Tunel 是检测细胞晚期凋亡的试剂。

Q:Annexin V 和JC-1、Tunel 细胞凋亡检测的可以应用到植物或是细菌(原核生物) 吗?

A:可以,但是需要制备原生质体,因为植物细胞或是细菌(原核生物)含有细胞壁,具体的染液使用剂量只需浸没细胞即可,染色时间对于不同细胞有一定的不同。

Q:40302ES Annexin V-FITC/PI 细胞凋亡检测试剂盒里的PI的浓度是多少呢?

A:20ug/ml。

Q:实验结果如何判断?

A:活细胞(Annexin V-/PI-)

 早期凋亡细胞(Annexin V+/PI-)

 晚期凋亡细胞和坏死细胞呈现双阳性(Annexin V+/PI+)

 裸核(Annexin V-/PI+)

Q: ​Annexin V和TUNEL有什么区别?

A:末端脱氧核苷酸转移酶 dUTP 缺口末端标记 (TUNEL) 是一种染色方法,用于识别细胞内 DNA 片段化位点——晚期细胞凋亡的标志性特征。 它使用酶末端脱氧核苷酸转移酶 (TdT) 将修饰的 dNTP(例如 dUTP)连接到片段化 DNA 链的 3'-羟基末端。 dNTPs 通常用荧光团修饰以促进量化和/或可视化。

Annexin V 染色通过结合由于细胞膜不对称性丧失而暴露在细胞外的 PS 残基来识别细胞凋亡的早期阶段。 Annexin V 通常用 FITC 等荧光团标记,以促进凋亡细胞的检测。

Q: ​1×Binding Buffer 的成分是什么?可以用什么替代?

A:该缓冲液的具体成分信息属于保密内容。如有替代需求,可选用PBS或Hanks缓冲液,但需补充添加2.5 mM CaCl₂,并将pH值调整至7.5。

Q: ​是否可以仅使用Annexin V-FITC进行检测而不检测PI?

A:可以的。对于特殊的检测样本,如红细胞无细胞核,如使用药物Doxorubicin(阿霉素)对PI检测存在干扰,对于这两种情况可以仅使用Annexin V-FITC进行检测。

Q: ​是否可以对组织切片进行染色(冰冻切片或石蜡切片)?

A:不可以。

Documents & Citations

Published citations

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