品牌翌圣生物 Yeasen
货号 / 型号93985ES
规格高活性、高纯度、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293细胞表达系统
应用方法
抗体药物靶点作为靶点蛋白用于抗体药物研发
适用物种种属
人
检测方法
ELISA
Tris-Bis PAGE
HPLC
LAL
生物特性参数
蛋白类型重组蛋白
种属来源人
生物活性描述ELISA Data: Immobilized Human MICA
His Tag at 1μg/ml (1μl/Well) on the plate. Dose response curve for Human NKG2D
hFc Tag with the EC5 of .88μg/ml determined by ELISA (QC Test).ELISA Data: Immobilized Human MICA
His Tag at 5μg/ml (1μl/well) on the plat
应用作为NKG2D配体,参与免疫应答
技术参数
内毒素水平< 1.0 EU per 1μg of the protein by the LAL method.
分子量The protein has a predicted MW of 35.8 kDa. Due to glycosylation
the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
纯度> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
储存条件-25~-15℃保存,收到货之后有效期1年。复溶后,2~8°C保存,7天有效期。复溶后,-85~-65℃保存,3-6个月有效期。
表达系统HEK293
标签C-His-Avi
外观冻干粉
复溶方法开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
Accession号Q96QC4
活性ELISA Data: Immobilized Human MICA
His Tag at 1μg/ml (1μl/Well) on the plate. Dose response curve for Human NKG2D
hFc Tag with the EC5 of .88μg/ml determined by ELISA (QC Test).ELISA Data: Immobilized Human MICA
His Tag at 5μg/ml (1μl/well) on the plat
实验类型
ELISA生物活性检测
LAL内毒素检测
Tris-Bis PAGE纯度检测
HPLC纯度检测