品牌翌圣生物 Yeasen
货号 / 型号93924ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293表达系统
应用方法
抗体药物靶点研究作为靶点蛋白用于抗体筛选和药物开发
适用物种种属
人
检测方法
SDS-PAGE
HPLC
LAL法
ELISA
生物特性参数
蛋白类型重组蛋白
种属来源人
生物活性描述【ELISA Data】 Immobilized Human Siglec-15
His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-15 Antibody
hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
应用抗体药物靶点研究
产品别称HsT1361
CD33L3
CD33 molecule-like 3
CD33 antigen-like 3
技术参数
纯度> 95% as determined by SDS-PAGE and HPLC.
内毒素水平< 1.0 EU per μg by the LAL method.
分子量The protein has a predicted MW of 29.5 kDa. Due to glycosylation
the protein migrates to 30-38 kDa based on SDS-PAGE result.
制剂形式Lyophilized from 0.22μm filtered solution in 20mM NaAC (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
活性Dose response curve for Anti-Siglec-15 Antibody
hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
储存条件-25 ~ -15℃保存,收到货之后有效期1年。复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期。
表达序列Phe20-Thr263
标签C-His-Avi
表达系统HEK293
外观Lyophilized Powder
Accession号Q6ZMC9
复溶方法Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAC (pH 5.0).
实验类型
SDS-PAGE纯度及分子量鉴定
HPLC纯度分析
LAL法内毒素检测
ELISA生物活性检测