品牌翌圣生物 Yeasen
货号 / 型号93805ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293表达系统
应用方法
抗体药物靶点蛋白靶点研究
适用物种种属
人
检测方法
Tris-Bis PAGE
HPLC
LAL法
ELISA
生物特性参数
蛋白类型重组蛋白
种属来源人
生物活性描述IGFBP-7 binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. ELISA Data: Immobilized Human IGFBP-7
His Tag at 5μg/ml (1μl/well) on the plate. Dose response curve for Human CD93
hFc Tag with the EC50 of 0.74μg/ml determined by ELISA.
应用靶点蛋白/抗体药物靶点蛋白
样本类型
重组蛋白
技术参数
纯度> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
内毒素水平< 1.0 EU per 1μg of the protein by the LAL method.
分子量预测分子量27.5 kDa；由于糖基化，Tris-Bis PAGE迁移至35-40 kDa.
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
储存条件-25~-15℃保存，收到货之后有效期1年。复溶后，2~8°C保存，7天有效期。复溶后，-85~-65℃保存，3-6个月有效期。
活性ELISA Data: Immobilized Human IGFBP-7
His Tag at 5μg/ml (1μl/well) on the plate. Dose response curve for Human CD93
hFc Tag with the EC50 of 0.74μg/ml determined by ELISA.
表达系统HEK293
标签N-His
表达序列Recombinant Human IGFBP-7 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Ser27-Leu282. [Accession | Q16270]
AccessionQ16270
外观冻干粉
产品别称IGFBP-7; IBP7; AGM; FSTL2; IGFBP-7v; IGFBPRP1; MAC25; PSF; RAMSVPS; TAF
复溶方法Centrifuge the tube before opening. Reconstituting to a concentration more than 1 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
实验类型
ELISA生物活性检测
HER2靶点蛋白：驱动肿瘤靶向治疗的核心力量
应用方向
细胞因子科学选型--常见问题解答
技术讲解
