品牌翌圣生物 Yeasen
货号 / 型号96848ES
纯度> 95% as determined by Bis-Tris PAGE> 95% as determined by H
规格25 μg
性状Lyophilized Powder
储存条件-25~-15℃保存,收到货之日起,有效期1年。复溶后, -85~-65℃保存,3个月有效期。避免反复冻融。
有效期1年
产地翌圣生物科技(上海)股份有限公司
复溶方法
用蒸馏水溶解冻干的蛋白。如果规格为25μg或者100μg,建议复溶到0.5mg/mL的浓度。对于500μg的冻干粉,建议以1mg/mL的浓度复溶。复溶过程中请不要剧烈涡旋或者剧烈摇晃。将复溶后的蛋白溶液按照需求进行分装,储存于至-80℃,避免反复冻融。
Accession
P24394-1
分子量
The protein has a predicted MW of 26.64 kDa. Due to glycosylation
the protein migrates to 58-68 kDa based on Bis-Tris PAGE result.
外观
Lyophilized Powder
产品别称
CD124; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124
制剂
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
纯度
> 95% as determined by Bis-Tris PAGE> 95% as determined by HPLC
表达序列
Recombinant Biotinylated Human IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Met26-His232
种属
Human
标签
C-His-Avi
内毒素
Less than 1 EU per μg by the LAL method.
表达系统
HEK293
技术平台
HEK293表达系统
应用方法
抗体药物靶点研究作为靶点蛋白用于药物筛选与功能研究
ELISA用于IL-4/IL-4R结合分析
适用物种种属
人
检测方法
Bis-Tris PAGE
HPLC
LAL
ELISA
生物特性参数
蛋白类型重组蛋白(IL-4Rα/CD124)
种属来源人
生物活性描述ELISA测定与人IL-4结合的EC50为6.4 ng/ml
应用ELISA检测;抗体药物靶点研究
技术参数
纯度>95% as determined by Bis-Tris PAGE; >95% as determined by HPLC
内毒素水平Less than 1 EU per μg by the LAL method
分子量The protein has a predicted MW of 26.64 kDa. Due to glycosylation
the protein migrates to 58-68 kDa based on Bis-Tris PAGE result.
制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
活性Dose response curve for Human IL-4
hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
储存条件-25~-15℃保存,收到货之日起有效期1年;复溶后-85~-65℃保存,3个月有效期;避免反复冻融。
表达系统HEK293
标签C-His-Avi
表达序列Recombinant Biotinylated Human IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Met26-His232.
外观Lyophilized Powder
Accession号P24394-1
复溶方法用蒸馏水溶解冻干的蛋白。如果规格为25μg或者100μg,建议复溶到0.5mg/mL的浓度。对于500μg的冻干粉,建议以1mg/mL的浓度复溶。复溶过程中请不要剧烈涡旋或者剧烈摇晃。将复溶后的蛋白溶液按照需求进行分装,储存于至-80℃,避免反复冻融。
产品别称CD124; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124
修饰Biotinylated
实验类型
ELISA测定蛋白与IL-4的结合活性
Bis-Tris PAGE检测蛋白纯度及分子量
HPLC检测蛋白纯度
LAL检测内毒素水平
有效期
1年
shelf_life
1年
可选规格
25 μg / 100 μg / 500 μg
规格
25 μg
单位
个
储存条件
-25~-15℃保存,收到货之日起,有效期1年。复溶后, -85~-65℃保存,3个月有效期。避免反复冻融。