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Tick the specs you need to submit them together; or enquire one by one. Cat. numbers are carried into the RFQ.
Q:荧光探针用什么缓冲液稀释？
A: 工作液用最好是使用无血清培养基、HBSS 或是 PBS 等缓冲液进行稀释，稀释后的探针工作液即可直接加入细胞进行检测。
Q: 几种探针的应用的位置？
A: 如果是测定胞质内的钙离子建议选择 Fluo-3、4，如果是测定细胞器内较浓钙离子的实验建议采用 Rhod-2。
Q: 用完该产品贴壁细胞均出现脱落现象怎么办？
A: 建议老师降低浓度。
Q:加探针孵育前要把细胞先消化下来吗？
A: 根据后续检测确定是否需要消化后孵育探针，如果是流式细胞仪或荧光酶标仪检测，可以先收集细胞再孵育探针。如果显微镜观察，可以不用消化。
Q:该试剂盒仅限于对胞质的检测吗？细胞器内可以吗？
A: 该探针检测的是细胞内总的钙离子水平，不具体区分细胞器。
Published citations
[1] Li Y, Xu X, Liu X, et al. Photoelectrons Mediating Angiogenesis and Immunotherapy through Heterojunction Film for Noninvasive Disinfection. Adv Sci (Weinh). 2020;7(17):2000023. Published 2020 Jul 25. doi:10.1002/advs.202000023(IF:15.840)
[2] Sun SC, Ma D, Li MY, et al. Mutations in C1orf194, encoding a calcium regulator, cause dominant Charcot-Marie-Tooth disease [published correction appears in Brain. 2019 Oct 1;142(10):e56]. Brain. 2019;142(8):2215-2229. doi:10.1093/brain/awz151(IF:11.814)
[3] Fu R, Xia Y, Li M, et al. Pim-1 as a Therapeutic Target in Lupus Nephritis. Arthritis Rheumatol. 2019;71(8):1308-1318. doi:10.1002/art.40863(IF:9.002)
[4] Wu L, Lin W, Liao Q, et al. Calcium Channel Blocker Nifedipine Suppresses Colorectal Cancer Progression and Immune Escape by Preventing NFAT2 Nuclear Translocation [published correction appears in Cell Rep. 2020 Dec 29;33(13):108582]. Cell Rep. 2020;33(4):108327. doi:10.1016/j.celrep.2020.108327(IF:8.109)
[5] Zhang Y, Zhou L, Liu Y, et al. A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans. Microbiol Spectr. 2022;10(1):e0082621. doi:10.1128/spectrum.00826-21(IF:7.171)
[6] Zeng Q, Yang J, Yan G, et al. Celastrol inhibits LL37-induced rosacea by inhibiting Ca2+/CaMKII-mTOR-NF-κB activation [published online ahead of print, 2022 Jun 16]. Biomed Pharmacother. 2022;153:113292. doi:10.1016/j.biopha.2022.113292(IF:6.530)
[7] Xu S, Cheng X, Wu J, et al. Capsaicin restores sodium iodine symporter-mediated radioiodine uptake through bypassing canonical TSH‒TSHR pathway in anaplastic thyroid carcinoma cells. J Mol Cell Biol. 2022;13(11):791-807. doi:10.1093/jmcb/mjab072(IF:6.216)
[8] Fu F, Wu D, Qian C. The MicroRNA-224 Inhibitor Prevents Neuronal Apoptosis via Targeting Spastic Paraplegia 7 After Cerebral Ischemia. J Mol Neurosci. 2016;59(3):421-429. doi:10.1007/s12031-016-0769-9(IF:2.352)
[9] Guo W, Wei B, Cheng T, Xu X, Ruan F, Xiang M. The Na+/K+ ATPase Inhibitor Ouabain Attenuates Stemness and Chemoresistance of Osteosarcoma Cells. Med Sci Monit. 2019;25:9426-9434. Published 2019 Dec 11. doi:10.12659/MSM.919266(IF:1.980)
