Hoechst 33342 Stain Solution (1 mg/mL) Hoechst 33342 染液(1 mg/mL)
勾选需要的规格可一次提交;也可以逐个询价。货号会一并带入询价单。
Q:染 A549 活细胞需要多少浓度的hoechst33342,多少浓度染多少时间最佳?
A:这个没有具体参考数据,可进行预实验,在推荐范围内调整。
Q:DAPI和Hoechst染料相当类似,如何二选一?
A:DAPI是非常通用的蓝色荧光染料,用于细胞核复染。对固定和透化的细胞/组织,具有非常明亮的细胞核标记。然而,此染料被认为是半渗透至不渗透的染料,用于活细胞染色的结合不一致。Hoechst 33342是一种细胞膜渗透性的染料,具有同DAPI的结合机制和荧光特征。非常适合用于活细胞成像,效果就如同DAPI用于固定细胞染色。
已发表文献
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[2] Wu X, Zhang X, Feng W, et al. A Targeted Erythrocyte Membrane-Encapsulated Drug-Delivery System with Anti-osteosarcoma and Anti-osteolytic Effects. ACS Appl Mater Interfaces. 2021;13(24):27920-27933. doi:10.1021/acsami.1c06059(IF:9.229)
[3] Zhong X, Bao X, Zhong H, et al. Mitochondrial targeted drug delivery combined with manganese catalyzed Fenton reaction for the treatment of breast cancer. Int J Pharm. 2022;622:121810. doi:10.1016/j.ijpharm.2022.121810(IF:5.875)
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[5] Jin L, Zheng D, Yang G, et al. Tilapia Skin Peptides Ameliorate Diabetic Nephropathy in STZ-Induced Diabetic Rats and HG-Induced GMCs by Improving Mitochondrial Dysfunction. Mar Drugs. 2020;18(7):363. Published 2020 Jul 15. doi:10.3390/md18070363(IF:5.118)
[6] Tian Y, Jiang Y, Li J, Wang D, Zhao H, Li Z. Effect of Stapling Architecture on Physiochemical Properties and Cell Permeability of Stapled α-Helical Peptides: A Comparative Study. Chembiochem. 2017;18(21):2087-2093. doi:10.1002/cbic.201700352(IF:2.847)
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