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Q：染 A549 活细胞需要多少浓度的hoechst33342，多少浓度染多少时间最佳？
A：这个没有具体参考数据，可进行预实验，在推荐范围内调整。
Q：DAPI和Hoechst染料相当类似，如何二选一？
A：DAPI是非常通用的蓝色荧光染料，用于细胞核复染。对固定和透化的细胞/组织，具有非常明亮的细胞核标记。然而，此染料被认为是半渗透至不渗透的染料，用于活细胞染色的结合不一致。Hoechst 33342是一种细胞膜渗透性的染料，具有同DAPI的结合机制和荧光特征。非常适合用于活细胞成像，效果就如同DAPI用于固定细胞染色。
Published citations
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[5] Jin L, Zheng D, Yang G, et al. Tilapia Skin Peptides Ameliorate Diabetic Nephropathy in STZ-Induced Diabetic Rats and HG-Induced GMCs by Improving Mitochondrial Dysfunction. Mar Drugs. 2020;18(7):363. Published 2020 Jul 15. doi:10.3390/md18070363(IF:5.118)
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