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Product data sheet PD-60316ES
ColitCare® Dextran Sulfate Sodium Salt(DSS)结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000 ColitCare® Dextran Sulfate Sodium Salt(DSS)结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000
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ColitCare® Dextran Sulfate Sodium Salt(DSS)结肠炎建模用葡聚糖硫酸钠盐 MW:36000~50000

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炎症性肠病研究
Product no.60316ES Sizes4 options Documents1 files SourceYeasen · Official
Cat. No. 60316ES25
现货 $1,058.00 促$1,255.00
Qty 1
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Specifications & Enquiry

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Cat.No Spec Price Status
60316ES25 25 g $1,058.00促$1,255.00 现货 Enquire
60316ES60 100 g $2,928.00促$3,855.00 现货 Enquire
60316ES76 500 g $10,628.00促$16,855.00 现货 Enquire
60316ES80 1 kg $20,528.00促$32,855.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No60316ES
Specification聚阴离子复合物,可溶于水,形成无色水溶液;纯度高,且具有良好的稳定性;可被自然降解。
Origin翌圣生物科技(上海)股份有限公司
应用方法 炎症性肠病研究用于研究溃疡性结肠炎等炎症性肠病的发病机制和药物评价
适用物种种属 小鼠 仓鼠
技术参数 CAS号9011-18-1 分子量36000~50000 溶解性易溶于水,微溶于乙醇 储存条件室温运输与保存,有效期2年 运输条件室温运输 外观白色或灰白色粉末 分子式(C6H7Na3O14S3) n 结构式https://upload.yeasen.com/file/4888/681e-172413764918137815.png 英文别名Dextran Sulfate Sodium Salt; DSS; Dextran Sodium sulfate 中文名称硫酸葡聚糖;葡聚糖硫酸氢钠;糖酐酯;硫酸葡聚糖钠盐 含硫量约17% 每个葡萄糖残糖的硫酸基团数平均1.9个
实验类型 DSS诱导结肠炎模型构建用于建立溃疡性结肠炎动物模型

FAQ

Q:针对结肠炎造模,每天给大鼠和小鼠饮水量体积是多少(包含急性和慢性造模)?

A:小鼠(20~25g)每只每天 7~10mL,大鼠(100 g)每只每天 10~11mL。

Q:小鼠急性和慢性造模DSS浓度是多少?造模周期是多久?

A:急性造模:3%~5%,周期:7天左右;慢性造模:1%~3%,周期:9周左右。给药浓度和动物类型、品系、体重大小以及给药方式都有关,需要客户参考相关文献并做预实验摸索最佳浓度。 另外预实验和正式实验所使用DSS批次尽可能保持一致,尽可能减少批次导致的实验干扰。

Q:3%的 DSS,小鼠饮用了三天,没有明显的体重下降现象?

A:这属于正常现象,小鼠基于个体差异,部分动物造模反应较慢,甚至饮用 DSS 第5天才出现体重下降的症状。若是第7天还未出现明显症状,建议客户提高 DSS 浓度到 3.5%~5%。

Q:结肠要造模,如何进行灌喂造模?

A:每天分别于 10:00,14:00和18:00 给予2%的 DSS 溶液灌喂,每次30mL/kg, 不需要另外补水。每次最后以一次灌喂后给予足量混合配方颗粒饲料,次日 8:00 取走饲料。连续造模 9 天即可。参考文献:葡聚糖硫酸钠自由饮用于定量灌喂诱导小鼠急性结肠炎模型的比较研究,胃肠病学 2009,14(1),27-30。

Q:结肠炎造模,造模失败的原因有哪些?

A:DSS造模会受到多种因素影响,包括小鼠类型、DSS分子量、DSS浓度、给药周期等。其中,DSS浓度是影响造模成功最主要的因素。造模失败可能的原因:DSS 浓度过低、给药时间短、小鼠未饮用到 DSS。

解决方案:

1、提高 DSS 浓度(有文献报道最高可用 10% DSS 给药),

2、延长给药周期,可持续给药 7-10 天,3、观察是否出现漏水、瓶口堵塞等情况。 Q:DSS为什么只有这个分子量的试剂可以造模?

A:DSS是一种常用于诱导结肠炎模型的化学物质。然而,不同分子量的DSS可能会产生不同的效果,因此只有特定分子量范围内的DSS适用于结肠炎模型。影响DSS适用性的关键因素是其溶解度和肠道渗透性。较低分子量的DSS溶解度较高,并且更容易通过肠道黏膜渗透,因此可以更好地模拟结肠炎的病理过程。较高分子量的DSS溶解度较低,并且渗透性较差,很难通过肠道黏膜进入肠壁,因此对于诱导结肠炎模型来说效果较差。因此,当选择适用于结肠炎模型的DSS时,需要考虑其分子量,以确保其溶解度和肠道渗透性与所需的病理模型相匹配。最适合结肠炎模型的DSS分子量可能因具体研究需求而不同,需要根据相关文献来选择合适的DSS。

Q:为什么造模选择60316ES这个产品,而其他的葡聚糖硫酸钠不可以用?

A:通常肠炎造模使用的分子量为36-50KD,小分子量的 DSS 致炎效果差,大分子量的 DSS 肠道不吸收。

Q: 从DSS诱导小鼠的粪便样本中提取DNA,结肠组织中提取的RNA中会有DSS残留,会抑制下游PCR,RT-PCR实验,如何处理?

A:精胺可以去除DSS对PCR的抑制。参考文献:Journal of Microbiological Methods ,2018 Jan, 144: 1-7。

Q:对于结肠炎造模,急性结肠炎造模,出现结肠没有变短,盲肠变大等现象是什么原因?

A:在动物结肠炎造模的过程中,慢性结肠炎的结肠变短很明显,急性结肠炎造模的结肠不是很明显,但是会出现结肠相对而言变脆,透明,充血等现象。建议客户通过HE切片实验结果来评价。急性结肠炎是结肠黏膜的急性炎症反应,盲肠变大可能是由于炎症引起的充血和水肿所致。

Documents & Citations

Published citations

客户使用该产品发表的高分科研文献(不完全统计)

2025

[1] J. Zhu, Y. Liu, T. Chen, et al. “Precision-Guided Killer” Engineered Phage for Combating Carbapenem-Resistant Klebsiella Pneumoniae Induced Inflammatory Bowel Disease. Adv. Funct. Mater. 2025, e00754. doi: 10.1002/adfm.202500754.IF=19

[2] Ma, X., Chen, J., Wang, F. et al. High fructose consumption aggravates inflammation by promoting effector T cell generation via inducing metabolic reprogramming. Sig Transduct Target Ther 10, 271 (2025). doi:10.1038/s41392-025-02359-9.IF=52.7

2024

[1] Zhong D, Jin K, Wang R, et al. Microalgae-Based Hydrogel for Inflammatory Bowel Disease and Its Associated Anxiety and Depression. Adv Mater. 2024 Jan 26:e2312275. doi: 10.1002/adma.202312275.IF=29.4

[2] Xuan Zhang, Xia Gao, Xiangzhou Yi,et al. Multi-targeting inulin-based nanoparticles with cannabidiol for effective prevention of ulcerative colitis[J]. Materials Today Bio.2024, 25100965. IF=8.2

2022

[1] Mengmeng Xu, Ying Kong, Nannan Chen,et al.Identification of Immune-Related Gene Signature and Prediction of CeRNA Network in Active Ulcerative Colitis[J].Frontiers in Immunology.2022; 13: 855645. IF=7.561

[2] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717

[3] Yuangeng Li,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451

[4] Wang S,  Huang J,  Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076

2021

[1] Li Zhao, Fei Wang,Zhengwei Cai,et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].chemical engineering journal.424(2021)130464.IF=16.744

[2] Lingjun Tong, Haining Hao, Zhe Zhang,et al.Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota[J].Theranostics.2021; 11(17): 8570-8586 IF=11.556

[3] Jingjing Gan, Yuxiao Liu, Lingyu Sun,et al.Orally administrated nucleotide-delivery particles from microfluidics for inflammatory bowel disease treatment[J].Applied Materials Today.2021 Dec;25:101231 IF=10.041

[4] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

[5] Hao H,  Zhang X,  Tong L,  Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From  Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147. IF=7.561

[6] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

2020

[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561

[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245

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