Hyaluronidase 透明质酸酶

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细胞分离

Product no.20426ES Sizes2 options Documents1 files SourceYeasen · Official

Cat. No. 20426ES60

现货 $186.00

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20426ES60 100 mg $186.00 现货 Enquire

20426ES80 1 g $1,286.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No20426ES

Specification100 mg

Origin翌圣生物科技(上海)股份有限公司

应用方法 细胞分离作为工具酶从组织分离活细胞

适用物种种属牛

生物特性参数 蛋白类型透明质酸酶（糖蛋白） 表达/来源系统牛睾丸 应用降解细胞外基质，从组织中分离活细胞

技术参数 CAS号37326-33-3 分子量~55 kDa 类型I-S 外观淡黄色至米黄色至褐色粉末 活性400–1 000 units/mg 最佳pH值4.5–6.0 抑制剂Fe2+，Fe3+，Zn2+，Cu2+，肝素，金精三羧酸，硫酸，硝酸，乙酰化透明质酸，硫酸纤维素酯，胆汁等 储存条件-20℃保存，两年有效 运输条件冰袋运输

实验类型 组织消化降解细胞外基质，从组织中分离活细胞

FAQ

Q：该酶的抑制剂有哪些？

A：Fe2+，Fe3+，Zn2+，Cu2+，肝素，金精三羧酸，硫酸，硝酸，乙酰化透明质酸，硫酸纤维素酯，胆汁等。

Q：该产品可以置于 4°吗？

A：一般-20℃保存。

Q：该工作液的缓冲液可以用磷酸盐吗？

A：可以。

Q：工作液可以保存多久？

A：该产品储存液不稳定，使用时建议现配现用。

Q：说明书中的溶液配制可以用商业化的PBS配制吗？

A：我们只能保证我们配方(含77mM氯化钠和0.01%BSA的磷酸盐buffer)是可以的，商业化的PBS一般氯化钠浓度会高一些，这个不有确定，没有测试过效果。

Q：当用于肿瘤组织的消化时有建议的透明质酸酶工作液浓度吗？

A：当用于肿瘤组织消化时，推荐工作浓度≥100 U/ml（以400 U/mg为例，浓度为0.25 mg/ml)，但不同批次对应的酶活会有不同，可提供批次号对酶活性数据进行确定。另可参考已发表的文献PMID:32001695（1 mg/ml），PMID:33625842（0.2 mg/ml）。

Documents & Citations

蛋白纯化产品目录 Open document

Published citations

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