rProtein A/G Agarose Resin 蛋白A/G琼脂糖纯化树脂
勾选需要的规格可一次提交；也可以逐个询价。货号会一并带入询价单。
Q：为什么柱子反压过高？
A：可能是填料被堵塞；裂解液中含有微笑的固体颗粒，建议上柱前使用 0.22 µm/0.45
µm 滤膜过滤。
Q：为什么洗脱组分中没有目的蛋白？
A：可能是抗体被降解；建议适当的提高洗脱pH。
Q：该产品可以直接使用吗？
A：Protein A 琼脂糖珠最好在使用前充分颠倒若干次，使琼脂糖珠混合均匀。
Q：该产品可以贮存在-20℃吗？
A：不可以，不可冷冻该产品。应置于 4°贮存。
Q：说明书中COIP实验操作中取适量填料是要加多少量的填料？
A：一般填料的量是根据自己要做的样品的量决定。最小的常用COIP体系为：20ul填料+5ug抗体与总体积500ul 总蛋白浓度1mg/ml的裂解液混合孵育。
Q：该产品可以重复使用吗？
A：用于偶连抗体进行蛋白的IP和coip不建议重复使用。
已发表文献
[1] Chen LJ, Zhang NN, Zhou CX, et al. Gm364 coordinates MIB2/DLL3/Notch2 to regulate female fertility through AKT activation. Cell Death Differ. 2022;29(2):366-380. doi:10.1038/s41418-021-00861-5(IF:15.828)
[2] Zhang K, Chen S, Yang Q, et al. The Oligodendrocyte Transcription Factor 2 OLIG2 regulates transcriptional repression during myelinogenesis in rodents [published correction appears in Nat Commun. 2022 Jun 1;13(1):3164]. Nat Commun. 2022;13(1):1423. Published 2022 Mar 17. doi:10.1038/s41467-022-29068-z(IF:14.919)
[3] Zhu D, Chen C, Liu X, et al. Osteosarcoma cell proliferation suppression via SHP-2-mediated inactivation of the JAK/STAT3 pathway by tubocapsenolide A. J Adv Res. 2021;34:79-91. Published 2021 Jun 11. doi:10.1016/j.jare.2021.06.004(IF:10.479)
[4] Zheng J, Yao L, Zhou Y, et al. A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis. Cell Death Dis. 2021;12(10):871. Published 2021 Sep 24. doi:10.1038/s41419-021-04159-9(IF:8.469)
[5] Wang J, Zhao D, Ding CZ, et al. MicroRNA-194: a novel regulator of glucagon-like peptide-1 synthesis in intestinal L cells. Cell Death Dis. 2021;12(1):113. Published 2021 Jan 21. doi:10.1038/s41419-020-03366-0(IF:8.469)
[6] Zhu H, Guo Y, Huang A, et al. HDAC3-Regulated PGE2 Production by Microglia Induces Phobic Anxiety Susceptibility After Stroke and Pointedly Exploiting a Signal-Targeted Gamma Visual Stimulation New Therapy. Front Immunol. 2022;13:845678. Published 2022 Feb 18. doi:10.3389/fimmu.2022.845678(IF:7.561)
[7] Wang Z, Xu J, Feng J, et al. Structural and Functional Analyses of Type I IFNa Shed Light Into Its Interaction With Multiple Receptors in Fish. Front Immunol. 2022;13:862764. Published 2022 Mar 22. doi:10.3389/fimmu.2022.862764(IF:7.561)
[8] Ni H, Qin H, Sun C, et al. MiR-375 reduces the stemness of gastric cancer cells through triggering ferroptosis. Stem Cell Res Ther. 2021;12(1):325. Published 2021 Jun 5. doi:10.1186/s13287-021-02394-7(IF:6.832)
[9] Gao L, Guo Q, Li X, et al. MiR-873/PD-L1 axis regulates the stemness of breast cancer cells [published correction appears in EBioMedicine. 2019 Nov;49:389-390]. EBioMedicine. 2019;41:395-407. doi:10.1016/j.ebiom.2019.02.034(IF:6.680)
[10] Yang X, Li X, Zhong C, et al. Circular RNA circPHKA2 Relieves OGD-Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR-574-5p to Modulate SOD2. Oxid Med Cell Longev. 2021;2021:3823122. Published 2021 Nov 8. doi:10.1155/2021/3823122(IF:6.543)
Protein A/G/L抗体亲和纯化的区别及选购指南
选购指南
Anti-Flag亲和纯化凝胶 ——IP/蛋白纯化好帮手
应用方向
Protein A/G琼脂糖或磁珠-抗体捕获神器，轻松搞定IP/COIP！
技术讲解
