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Product data sheet PD-36403ES

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In stock Protein Purification 翌圣生物 Yeasen

rProtein A/G Agarose Resin 蛋白A/G琼脂糖纯化树脂

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亲和层析抗体纯化蛋白功能研究

Product no.36403ES Sizes5 options Documents1 files SourceYeasen · Official

Cat. No. 36403ES03

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Cat.No Spec Price Status

36403ES03 1 mL $268.00促~~$278.00~~ 现货 Enquire

36403ES05 2 mL $518.00促~~$538.00~~ 现货 Enquire

36403ES08 5 mL $838.00促~~$878.00~~ 现货 Enquire

36403ES25 25 mL $2,748.00促~~$2,886.00~~ 现货 Enquire

36403ES60 100 mL $7,058.00促~~$7,426.00~~ 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No36403ES

Specification基质（Matrix）高度交联的4%琼脂糖微球配体（Ligand）重组蛋白A/G孔径（Bead size）45-165 µm最大流速（Flowmax）0.3 MPa, 3 bar储存缓冲液（Buffer）含20%乙醇的1×PBS载量（Capa

Origin翌圣生物科技(上海)股份有限公司

技术平台 亲和层析

应用方法 抗体纯化分离纯化单克隆和多克隆抗体 蛋白功能研究通过免疫沉淀/免疫共沉淀进行蛋白功能相关研究

适用物种种属通用

检测方法通用

生物特性参数 蛋白类型重组蛋白A/G 来源生物金黄色葡萄球菌（Protein A）、链球菌属（Protein G） 结合靶标免疫球蛋白(Ig)Fc区，主要结合大多数哺乳动物IgG 应用抗体分离纯化；免疫沉淀（IP）；免疫共沉淀（Co-IP）

样本类型通用

技术参数 制剂配方含20%乙醇的1×PBS；凝胶与保护液比例为1:1 基质高度交联的4%琼脂糖微球 配体重组蛋白A/G 粒径45-165 µm 最大流速0.3 MPa 3 bar 活性/载量约10-15 mg Rabbit IgG/mL基质 pH范围3-10 储存条件2~8℃保存，有效期2年

实验类型 亲和层析组装层析柱或批式吸附，用于抗体纯化 IP用于蛋白功能相关研究 Co-IP用于蛋白相互作用及功能研究

FAQ

Q：为什么柱子反压过高？

A：可能是填料被堵塞；裂解液中含有微笑的固体颗粒，建议上柱前使用 0.22 µm/0.45

µm 滤膜过滤。

Q：为什么洗脱组分中没有目的蛋白？

A：可能是抗体被降解；建议适当的提高洗脱pH。

Q：该产品可以直接使用吗？

A：Protein A 琼脂糖珠最好在使用前充分颠倒若干次，使琼脂糖珠混合均匀。

Q：该产品可以贮存在-20℃吗？

A：不可以，不可冷冻该产品。应置于 4°贮存。

Q：说明书中COIP实验操作中取适量填料是要加多少量的填料？

A：一般填料的量是根据自己要做的样品的量决定。最小的常用COIP体系为：20ul填料+5ug抗体与总体积500ul 总蛋白浓度1mg/ml的裂解液混合孵育。

Q：该产品可以重复使用吗？

A：用于偶连抗体进行蛋白的IP和coip不建议重复使用。

Documents & Citations

蛋白纯化产品目录 Open document

Published citations

[1] Chen LJ, Zhang NN, Zhou CX, et al. Gm364 coordinates MIB2/DLL3/Notch2 to regulate female fertility through AKT activation. Cell Death Differ. 2022;29(2):366-380. doi:10.1038/s41418-021-00861-5(IF:15.828)

[2] Zhang K, Chen S, Yang Q, et al. The Oligodendrocyte Transcription Factor 2 OLIG2 regulates transcriptional repression during myelinogenesis in rodents [published correction appears in Nat Commun. 2022 Jun 1;13(1):3164]. Nat Commun. 2022;13(1):1423. Published 2022 Mar 17. doi:10.1038/s41467-022-29068-z(IF:14.919)

[3] Zhu D, Chen C, Liu X, et al. Osteosarcoma cell proliferation suppression via SHP-2-mediated inactivation of the JAK/STAT3 pathway by tubocapsenolide A. J Adv Res. 2021;34:79-91. Published 2021 Jun 11. doi:10.1016/j.jare.2021.06.004(IF:10.479)

[4] Zheng J, Yao L, Zhou Y, et al. A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis. Cell Death Dis. 2021;12(10):871. Published 2021 Sep 24. doi:10.1038/s41419-021-04159-9(IF:8.469)

[5] Wang J, Zhao D, Ding CZ, et al. MicroRNA-194: a novel regulator of glucagon-like peptide-1 synthesis in intestinal L cells. Cell Death Dis. 2021;12(1):113. Published 2021 Jan 21. doi:10.1038/s41419-020-03366-0(IF:8.469)

[6] Zhu H, Guo Y, Huang A, et al. HDAC3-Regulated PGE2 Production by Microglia Induces Phobic Anxiety Susceptibility After Stroke and Pointedly Exploiting a Signal-Targeted Gamma Visual Stimulation New Therapy. Front Immunol. 2022;13:845678. Published 2022 Feb 18. doi:10.3389/fimmu.2022.845678(IF:7.561)

[7] Wang Z, Xu J, Feng J, et al. Structural and Functional Analyses of Type I IFNa Shed Light Into Its Interaction With Multiple Receptors in Fish. Front Immunol. 2022;13:862764. Published 2022 Mar 22. doi:10.3389/fimmu.2022.862764(IF:7.561)

[8] Ni H, Qin H, Sun C, et al. MiR-375 reduces the stemness of gastric cancer cells through triggering ferroptosis. Stem Cell Res Ther. 2021;12(1):325. Published 2021 Jun 5. doi:10.1186/s13287-021-02394-7(IF:6.832)

[9] Gao L, Guo Q, Li X, et al. MiR-873/PD-L1 axis regulates the stemness of breast cancer cells [published correction appears in EBioMedicine. 2019 Nov;49:389-390]. EBioMedicine. 2019;41:395-407. doi:10.1016/j.ebiom.2019.02.034(IF:6.680)

[10] Yang X, Li X, Zhong C, et al. Circular RNA circPHKA2 Relieves OGD-Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR-574-5p to Modulate SOD2. Oxid Med Cell Longev. 2021;2021:3823122. Published 2021 Nov 8. doi:10.1155/2021/3823122(IF:6.543)