Bradford Protein Quantification Kit Bradford蛋白浓度测定试剂盒

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比色皿法酶标法蛋白定量

Product no.20202ES Sizes2 options Documents1 files SourceYeasen · Official

Cat. No. 20202ES76

现货 $98.00

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Cat.No Spec Price Status

20202ES76 500 T（125 mL） $98.00 现货 Enquire

20202ES86 2500 T（625 mL） $398.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No20202ES

Specification1. 大多数化学物质的兼容性比较好，比如对还原剂DTT的兼容性高达5mM。2.灵敏度高，最低蛋白检测量可达1μg。

Origin翌圣生物科技(上海)股份有限公司

技术平台 比色皿法 酶标法

应用方法 蛋白定量用于Western Blot等蛋白分析实验的样本定量

检测方法 分光光度法

生物特性参数 蛋白类型牛血清白蛋白(BSA) 来源系统牛血清 应用蛋白定量

技术参数浓度2 mg/mL（蛋白标准品BSA） 灵敏度最低蛋白检测量1μg 储存条件染色液4℃保存；蛋白标准品常温或4℃保存 运输条件冰袋运输 保质期1年规格比色皿法125次/625次；酶标法500次/2500次 兼容性对还原剂DTT的兼容性高达5mM

实验类型 Bradford蛋白浓度测定法利用考马斯亮蓝G-250与蛋白结合测定蛋白浓度

FAQ

Q：该产品与 20201 有什么区别？

A：该产品灵敏度低；易受TritonX-100、SDS 等去污剂的影响；作标曲时，线性比较差。

Q：Bradord 其中主要组分的颜色？

A：A 液的颜色为淡蓝色，不是其他的颜色。

Q：该产品如何储存？

A：染色液 4℃保存，蛋白标准品常温或 4℃保存。

Q：Bradford 染色液可以直接使用吗？

A：Bradford 染色液需恢复到室温再使用，有利于提高检测的灵敏度。另，使用前需颠倒几次以充分混匀。

Documents & Citations

Western Blot产品册 Open document

Published citations

Shi W, Sun S, Liu H, et al. Guiding bar motif of thioredoxin reductase 1 modulates enzymatic activity and inhibitor binding by communicating with the co-factor FAD and regulating the flexible C-terminal redox motif. Redox Biol. 2024;70:103050. doi:10.1016/j.redox.2024.103050(IF:11.4)
Zhao W, Li J, Wang X, et al. Prime editor-mediated functional reshaping of ACE2 prevents the entry of multiple human coronaviruses, including SARS-CoV-2 variants. MedComm (2020). 2023;4(5):e356. Published 2023 Sep 10. doi:10.1002/mco2.356(IF:9.9)
Jin Q, Pan F, Hu CF, Lee SY, Xia XX, Qian ZG. Secretory production of spider silk proteins in metabolically engineered Corynebacterium glutamicum for spinning into tough fibers. Metab Eng. 2022;70:102-114. doi:10.1016/j.ymben.2022.01.009(IF:9.783)
Zhao H, Wei J, Du Y, et al. Improved cognitive impairments by silencing DMP1 via enhancing the proliferation of neural progenitor cell in Alzheimer-like mice. Aging Cell. 2022;21(5):e13601. doi:10.1111/acel.13601(IF:9.304)
Li Y, Yang X, Zhang J, et al. Ketogenic Diets Induced Glucose Intolerance and Lipid Accumulation in Mice with Alterations in Gut Microbiota and Metabolites. mBio. 2021;12(2):e03601-20. Published 2021 Mar 30. doi:10.1128/mBio.03601-20(IF:7.867)
Li J, Wen S, Li B, Li N, Zhan X. Phosphorylation-Mediated Molecular Pathway Changes in Human Pituitary Neuroendocrine Tumors Identified by Quantitative Phosphoproteomics. Cells. 2021;10(9):2225. Published 2021 Aug 27. doi:10.3390/cells10092225(IF:6.600)
Wen S, Li J, Yang J, Li B, Li N, Zhan X. Quantitative Acetylomics Revealed Acetylation-Mediated Molecular Pathway Network Changes in Human Nonfunctional Pituitary Neuroendocrine Tumors. Front Endocrinol (Lausanne). 2021;12:753606. Published 2021 Oct 12. doi:10.3389/fendo.2021.753606(IF:5.555)
Pan Y, Bao J, Zhang X, et al. Rational Design of P450 aMOx for Improving Anti-Markovnikov Selectivity Based on the "Butterfly" Model. Front Mol Biosci. 2022;9:888721. Published 2022 May 23. doi:10.3389/fmolb.2022.888721(IF:5.246)