HiActi® Recombinant Bovine IL-6 Protein 重组牛白介素-6

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CHO细胞表达系统基于细胞的检测

Product no.90365ES Sizes3 options Documents1 files SourceYeasen · Official

Cat. No. 90365ES10

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90365ES10 10 μg $1,185.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No90365ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 CHO细胞表达系统

应用方法 基于细胞的检测评估重组蛋白的生物活性

适用物种种属牛

检测方法 SDS-PAGE 凝胶法 细胞增殖实验

生物特性参数 蛋白类型重组牛IL-6蛋白（细胞因子） 种属来源牛（Bovine） 生物活性描述Measured in a cell proliferation assay using M-NFS-60 cells. The ED50 for this effect is ≤0.1 ng/mL. 应用Cell Based Assay（基于细胞的检测）用于测定生物活性

技术参数纯度≥ 95% as analyzed by SDS-PAGE 内毒素水平< 10 EU/mg of protein by gel clotting method 分子量~ 20.7 kDa 制剂形式Lyophilized from a 0.22 μm filtered solution containing PBS 5% Mannitol and 0.01% Tween 80 pH 7.4. 储存条件-85 ~ -65℃保存，收到货之后有效期6个月。复溶后，产品在4°C下稳定2周，在-20°C下稳定3个月。避免反复冻融循环。 表达系统CHO 标签No Tag 外观Lyophilized Powder 复溶方法使用无菌去离子水复溶，重新配制至0.1-1.0 mg/mL的浓度，可根据后续实验更进一步稀释 表达序列Gly30-Lys208 Accession号P26892 活性ED50 ≤0.1 ng/mL