Recombinant Mouse IL-17A&F Protein,Strep-II, N-His Tag

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HEK293细胞表达系统炎症研究免疫学研究

Product no.96834ES Sizes3 options SourceYeasen · Official

Cat. No. 96834ES25

现货 ¥1,355.00

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96834ES25 25 μg ¥1,355.00 现货 Enquire

96834ES60 100 μg ¥4,945.00 现货 Enquire

96834ES76 500 μg ¥16,495.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No96834ES

Specification高活性、高纯度、低内毒素、高稳定性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 炎症研究IL-17A在慢性炎症中发挥重要作用 免疫学研究细胞因子与免疫调节研究

适用物种种属小鼠

检测方法 Bis-Tris PAGE HPLC LAL法 ELISA

生物特性参数 蛋白类型重组细胞因子 种属来源Mouse 生物活性描述IL-17A在抗微生物和慢性炎症中起重要作用，是IL17RA和IL17RC的配体；IL-17A/IL-17F异二聚体是IL17RA/IL17RC异二聚体复合物的配体，参与诱导基质细胞产生促炎和造血细胞因子。应用用于炎症和免疫学研究，ELISA检测

技术参数 内毒素水平Less than 0.1 EU per μg by the LAL method. 纯度> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC 分子量Predicted MW 16.01 kDa (IL-17A) and 15.98 kDa (IL-17F); due to glycosylation migrates to 13-23 kDa and 25-35 kDa by Bis-Tris PAGE 制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 储存条件-25~-15℃保存，收到货之日起，有效期1年。复溶后，-85~-65℃保存，3个月有效期。避免反复冻融。 表达系统HEK293 标签N-Strep-II N-His 复溶方法用蒸馏水溶解冻干的蛋白。如果规格为25μg或者100μg，建议复溶到0.5mg/mL的浓度。对于500μg的冻干粉，建议以1mg/mL的浓度复溶。复溶过程中请不要剧烈涡旋或者剧烈摇晃。将复溶后的蛋白溶液按照需求进行分装，储存于至-80℃，避免反复冻融。 表达序列Recombinant Mouse IL-17A&F Protein is expressed as a heterodimer of IL-17A and IL-17F by co-expression in HEK293. Subunit IL-17A is fused with a Strep II tag at the N-terminus and subunit IL-17F is fused with a His tag at the N-terminus. It contains Ala26-Ala158 (IL-17A) and Arg29-Ala161 (IL-17F). AccessionQ62386(IL-17A)&Q7TNI7-1(IL-17F) 外观Lyophilized Powder