2× Hieff® PCR Master Mix (With Dye)
勾选需要的规格可一次提交；也可以逐个询价。货号会一并带入询价单。
Q：PCR Mix 可以用于 PAGE 电泳吗?
A：不建议，PCR Mx 中含有影响 PAGE 胶电泳的组分。推荐客户使用 10101
Q：PCR Mix 可以减少体系么？
A：可以，使用的引物、模板等比减少使用。
Q：10102 和 10103 有什么区别?
A：区别在于 10102 预混有溴酚蓝指示剂。后续可直接进行凝胶电泳。而 10103 中无溴酚蓝指示剂，电泳时需要添加DNA loading buffer。
Q：扩增完送测序。应选有染料的还是无染料?
A：都可以。PCR 产物测序，测序公司会先进行纯化。
Q：10102 镁离子浓度，dNTP 浓度分别是多少？
A：镁离子终浓度 2mM，200 µM of each dNTP。
Q：PCR Mix 扩增完成后,可以直接做酶切吗?
A：不建议，某些组分可能会影响酶切效果。
Q：PCR Mix 有没有 3’-5’的外切酶活性？PCR Mix 扩增产物类型？
A：没有 3’-5’的外切酶活性，产物带有 3’-A。可用于TA 克隆。
已发表文献
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[2] Li Y, Wang C, Zhang L, Chen B, Mo Y, Zhang J. Claudin-5a is essential for the functional formation of both zebrafish blood-brain barrier and blood-cerebrospinal fluid barrier. Fluids Barriers CNS. 2022;19(1):40. Published 2022 Jun 3. doi:10.1186/s12987-022-00337-9(IF:7.662)
[3] Luo T, Wang Y, Tang H, et al. An AAV-Based NF-κB-Targeting Gene Therapy (rAAV-DMP-miR533) to Inflammatory Diseases. J Inflamm Res. 2022;15:3447-3466. Published 2022 Jun 14. doi:10.2147/JIR.S362732(IF:6.922)
[4] Chen Z, Qi Z, He D, et al. Strategy for Scanning Peptide-Coding Circular RNAs in Colorectal Cancer Based on Bioinformatics Analysis and Experimental Assays. Front Cell Dev Biol. 2022;9:815895. Published 2022 Feb 25. doi:10.3389/fcell.2021.815895(IF:6.684)
[5] Yu Y, Fang L. CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer. Cell Death Discov. 2022;8(1):152. Published 2022 Apr 2. doi:10.1038/s41420-022-00965-y(IF:5.241)
[6] Zhang J, Liu W, Li G, et al. BCAS2 is involved in alternative splicing and mouse oocyte development. FASEB J. 2022;36(2):e22128. doi:10.1096/fj.202101279R(IF:5.192)
[7] Xiao W, Li J, Zhang Y, et al. A fungal Bipolaris bicolor strain as a potential bioherbicide for goosegrass (Eleusine indica) control. Pest Manag Sci. 2022;78(3):1251-1264. doi:10.1002/ps.6742(IF:4.845)
[8] Zhang Y, Yu R, Tang J, et al. Three cytochrome P450 CYP4 family genes regulated by the CncC signaling pathway mediate phytochemical susceptibility in the red flour beetle, Tribolium castaneum. Pest Manag Sci. 2022;78(8):3508-3518. doi:10.1002/ps.6991(IF:4.845)
[9] Zhang X, Yang S, Chen W, et al. Circular RNA circYPEL2: A Novel Biomarker in Cervical Cancer. Genes (Basel). 2021;13(1):38. Published 2021 Dec 23. doi:10.3390/genes13010038(IF:4.096)
[10] Gu K, Qian D, Qin H, et al. A novel mutation in KCNH2 yields loss-of-function of hERG potassium channel in long QT syndrome 2. Pflugers Arch. 2021;473(2):219-229. doi:10.1007/s00424-021-02518-1(IF:3.657)
[11] Yang Y, Chu X, Nie M, et al. A novel long-range deletion spanning STX16 and NPEPL1 causing imprinting defects of the GNAS locus discovered in a patient with autosomal-dominant pseudohypoparathyroidism type 1B. Endocrine. 2020;69(1):212-219. doi:10.1007/s12020-020-02304-6(IF:3.235)
[12] Ullah H, Arbab S, Khan MIU, et al. Circulating cell-free mitochondrial DNA fragment: A possible marker for early detection of Schistosoma japonicum. Infect Genet Evol. 2021;88:104683. doi:10.1016/j.meegid.2020.104683(IF:2.773)
[13] Xu L, Chen Y, Shen T, Lin C, Zhang B. Genetic Analysis of PICK1 Gene in Alzheimer's Disease: A Study for Finding a New Gene Target. Front Neurol. 2019;9:1169. Published 2019 Jan 9. doi:10.3389/fneur.2018.01169(IF:2.635)
[14] Ullah H, Qadeer A, Giri BR. Detection of circulating cell-free DNA to diagnose Schistosoma japonicum infection. Acta Trop. 2020;211:105604. doi:10.1016/j.actatropica.2020.105604(IF:2.555)
[15] Yan ZC, Hua HQ, Qi GY, Li YX. Early Detection and Identification of Parasitoid Wasps Trichogramma Westwood (Hymenoptera: Trichogrammatidae) in Their Host Eggs Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism [published online ahead of print, 2022 Jun 24]. J Econ Entomol. 2022;toac095. doi:10.1093/jee/toac095(IF:2.381)
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翌圣高保真DNA聚合酶 解决您的PCR扩增难题
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