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Product data sheet PD-92207ES
HiActi® Recombinant Human IGF-2 Protein 重组人胰岛素样生长因子-2 HiActi® Recombinant Human IGF-2 Protein 重组人胰岛素样生长因子-2
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HiActi® Recombinant Human IGF-2 Protein 重组人胰岛素样生长因子-2

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细胞培养干细胞分化研究发育和再生模型构建
Product no.92207ES Sizes3 options SourceYeasen · Official
Cat. No. 92207ES10
现货 $815.00
Qty 1
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Cat.No Spec Price Status
92207ES10 10 μg $815.00 现货 Enquire
92207ES60 100 μg $3,665.00 现货 Enquire
92207ES50 50 μg $2,545.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No92207ES
Specification高纯度、高活性、低内毒素、高批间一致性
Origin翌圣生物科技(上海)股份有限公司
应用方法 细胞培养作为培养基添加成分,支持原代细胞、干细胞或肿瘤细胞体外的生长与存活研究 干细胞分化研究用于诱导和优化多能干细胞、骨骼肌细胞或成骨细胞的分化方案 发育和再生模型构建用于构建发育和再生模型
适用物种种属 人
检测方法 SDS-PAGE HPLC LAL法
生物特性参数 蛋白类型重组人胰岛素样生长因子-2 种属来源人 生物活性描述Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/mL corresponding to a specific activity of > 5.0 × 10^5 IU/mg.
样本类型 重组蛋白
技术参数 纯度>98% as determined by SDS-PAGE and HPLC 内毒素水平<1.0 EU/μg by LAL method 分子量Approximately 7.5 kDa 制剂形式Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl pH 8.0 150 mM NaCl with 0.02% Tween-20 活性ED50 < 2 ng/mL; specific activity > 5.0 × 10^5 IU/mg 储存条件-25~-15℃保存,有效期1年;复溶后,2~8℃保存7天;-85~-65℃保存3个月 表达系统E.coli 标签No Tag 表达序列Ala25-Glu91 复溶方法开盖之前,先离心,以将内容物带到底部。使用无菌蒸馏水或PBS复溶,重新配制至0.1-1.0 mg/mL的浓度,可根据后续实验更进一步稀释;长期储存,建议稀释缓冲液中加入载体蛋白(0.1% BSA);分装为一次实验用量,-80℃冻存,避免反复冻融。 外观Lyophilized Powder 蛋白登录号E3UN46
实验类型 细胞增殖实验用于测定生物活性

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