Recombinant Human TIGIT (His Tag)

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HEK293表达系统免疫学研究

Product no.93561ES Sizes4 options SourceYeasen · Official

Cat. No. 93561ES20

现货 ¥985.00

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Cat.No Spec Price Status

93561ES20 20 μg ¥985.00 现货 Enquire

93561ES60 100 μg ¥4,445.00 现货 Enquire

93561ES76 500 μg ¥14,855.00 现货 Enquire

93561ES80 1 mg ¥28,225.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93561ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 免疫学研究研究TIGIT介导的T细胞和NK细胞调节

适用物种种属人

检测方法 ELISA SPR SDS-PAGE HPLC LAL

生物特性参数 蛋白类型重组蛋白 种属来源Human 生物活性描述1.【ELISA Data】Immobilized Human TIGIT His Tag at 0.5μg/mL(100μL/well) on the plate. Dose response curve for Anti-TIGIT Antibody hFc Tag with the EC50 of 18ng/mL determined by ELISA. 2.【SPR Data】Human CD155 hFc Tag captured on Protein A chip can bind Human TIGIT His Tag with an affinity constant of 0.17μM as determined in a SPR assay. 应用免疫检查点研究，免疫调节研究

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平≤ 1.0 EU per μg by the LAL method. 分子量Approximately 14 kDa. Due to glycosylation the protein migrates to 20-25 kDa based on Tris-Bis PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 活性EC50 of 18ng/mL determined by ELISA; affinity constant of 0.17μM determined by SPR. 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达序列Met22-Pro141 表达系统HEK293 标签C-His 外观Lyophilized Powder 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 AccessionQ495A1-1