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荧光定量PCR基因定量分析基因分型

Product no.11208ES Sizes3 options Documents1 files SourceYeasen · Official

Cat. No. 11208ES03

现货 $1,365.00

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Cat.No Spec Price Status

11208ES03 1 mL $1,365.00 现货 Enquire

11208ES08 5 mL $5,565.00 现货 Enquire

11208ES60 20× 5 mL $91,265.00 现货 Enquire

Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No11208ES

Specification灵敏度高

Origin翌圣生物科技(上海)股份有限公司

技术平台 荧光定量PCR

应用方法 基因定量分析用于基因多重定量分析 基因分型用于基因分型研究

适用物种种属通用

检测方法 探针法

生物特性参数 试剂类型TaqMan多重qPCR预混液 应用探针法多重荧光定量PCR

样本类型通用

技术参数 产品形式2×预混合试剂 储存条件-20°C储存 运输条件冰袋运输 有效期18个月 热启动抗体法热启动 适配型号大多数荧光定量机型 酶基因改造的热启动Taq酶

实验类型 多重荧光定量PCR在单个反应孔中进行多重目标基因定量检测 基因分型用于样本基因分型

FAQ

Q：11208 跟 11205 有什么区别？

A：酶不一样，11208 是可以做多重PCR，11205 只能做单重PCR，做qPCR 是没有问题的。

Q：11208 的qPCR 反应体系可以用 5 μL/10 μL 吗？

A：可以，但一般推荐 20ul。大体系减少误差。5μl 体系太小，加样误差会很大，不推荐。

Q：11208 可扩增多长的产物啊？

A：建议在 300bp 以下，过长的产物会导致酶效降低。若一定要用，建议用三步法。

Documents & Citations

实时荧光定量PCR实验技术手册(反转定量) Open document

Published citations

[1] Han S, Cao D, Sha J, Zhu X, Chen D. LncRNA ZFPM2-AS1 promotes lung adenocarcinoma progression by interacting with UPF1 to destabilize ZFPM2. Mol Oncol. 2020;14(5):1074-1088. doi:10.1002/1878-0261.12631(IF:6.574)

[2] Zhang Y, Fang Z, Guo X, et al. lncRNA B4GALT1-AS1 promotes colon cancer cell stemness and migration by recruiting YAP to the nucleus and enhancing YAP transcriptional activity. J Cell Physiol. 2019;234(10):18524-18534. doi:10.1002/jcp.28489(IF:4.522)

[3] Li H, Liu F, Qin W. Circ_0072083 interference enhances growth-inhibiting effects of cisplatin in non-small-cell lung cancer cells via miR-545-3p/CBLL1 axis. Cancer Cell Int. 2020;20:78. Published 2020 Mar 12. doi:10.1186/s12935-020-1162-x(IF:4.175)

[4] Zhao W, Wu M, Cui L, Du W. Limonin attenuates the stemness of cervical carcinoma cells by promoting YAP nuclear-cytoplasmic translocation. Food Chem Toxicol. 2019;125:621-628. doi:10.1016/j.fct.2019.02.011(IF:3.775)

[5] Cao X, Zhang C, Zhang X, Chen Y, Zhang H. MiR-145 negatively regulates TGFBR2 signaling responsible for sepsis-induced acute lung injury. Biomed Pharmacother. 2019;111:852-858. doi:10.1016/j.biopha.2018.12.138(IF:3.743)

[6] Su Z, Wang C, Chang D, Zhu X, Sai C, Pei J. Limonin attenuates the stemness of breast cancer cells via suppressing MIR216A methylation. Biomed Pharmacother. 2019;112:108699. doi:10.1016/j.biopha.2019.108699(IF:3.743)

[7] Zhang C, Li J, Qiu X, Chen Y, Zhang X. SUMO protease SENP1 acts as a ceRNA for TGFBR2 and thus activates TGFBR2/Smad signaling responsible for LPS-induced sepsis. Biomed Pharmacother. 2019;112:108620. doi:10.1016/j.biopha.2019.108620(IF:3.743)

[8] Ma F, Li Z, Cao J, Kong X, Gong G. A TGFBR2/SMAD2/DNMT1/miR-145 negative regulatory loop is responsible for LPS-induced sepsis. Biomed Pharmacother. 2019;112:108626. doi:10.1016/j.biopha.2019.108626(IF:3.743)

[9] Guo J, Zhang L, Lian L, Hao M, Chen S, Hong Y. CircATP2B4 promotes hypoxia-induced proliferation and migration of pulmonary arterial smooth muscle cells via the miR-223/ATR axis. Life Sci. 2020;262:118420. doi:10.1016/j.lfs.2020.118420(IF:3.647)

[10] Song H, Xu Y, Shi L, et al. LncRNA THOR increases the stemness of gastric cancer cells via enhancing SOX9 mRNA stability. Biomed Pharmacother. 2018;108:338-346. doi:10.1016/j.biopha.2018.09.057(IF:3.457)

[11] Lou W, Yan J, Wang W. Downregulation of miR-497-5p Improves Sepsis-Induced Acute Lung Injury by Targeting IL2RB. Biomed Res Int. 2021;2021:6624702. Published 2021 Apr 12. doi:10.1155/2021/6624702(IF:3.411)

[12] Song H, Shi L, Xu Y, et al. BRD4 promotes the stemness of gastric cancer cells via attenuating miR-216a-3p-mediated inhibition of Wnt/β-catenin signaling. Eur J Pharmacol. 2019;852:189-197. doi:10.1016/j.ejphar.2019.03.018(IF:3.170)