Recombinant SARS-COV-2 Spike RBD Protein,mFc Tag

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HEK293表达系统病毒感染机制研究

Product no.96465ES Sizes3 options SourceYeasen · Official

Cat. No. 96465ES25

现货 $1,135.00

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Cat.No Spec Price Status

96465ES25 25 μg $1,135.00 现货 Enquire

96465ES60 100 μg $4,505.00 现货 Enquire

96465ES76 500 μg $15,005.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No96465ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 病毒感染机制研究研究S蛋白RBD与ACE2受体的结合

适用物种种属 SARS-CoV-2

检测方法 ELISA SDS-PAGE SEC-HPLC Bis-Tris PAGE LAL法

生物特性参数 蛋白类型重组SARS-CoV-2 Spike RBD蛋白 种属来源SARS-CoV-2 生物活性ELISA Data 1: Immobilized SARS-COV-2 Spike RBD at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2 hFc Tag with the EC50 of 11.6ng/ml determined by ELISA. 应用用于研究S蛋白与ACE2受体的结合

技术参数纯度> 95% as determined by SDS-PAGE and SEC-HPLC 内毒素水平Less than 1 EU per μg by the LAL method. 分子量The protein has a predicted MW of 49.7 kDa. Due to glycosylation the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 储存条件-25~-15℃保存，有效期1年；复溶后-85~-65℃保存，3个月；避免反复冻融。 表达系统HEK293 标签C-mFc 外观Lyophilized Powder 表达序列Arg319-Asn532 复溶方法用蒸馏水溶解冻干的蛋白。如果规格为25μg或者100μg，建议复溶到0.5mg/mL的浓度。对于500μg或1mg的冻干粉，建议以1mg/mL的浓度复溶。复溶过程中请不要剧烈涡旋或者剧烈摇晃。将复溶后的蛋白溶液按照需求进行分装，储存于至-80℃，避免反复冻融。活性EC50=11.6ng/ml (ELISA) 登录号QHD43416.1