Recombinant SARS-COV-2 Spike S1 (N501Y) Protein,His Tag

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HEK293表达系统COVID-19研究

Product no.96472ES Sizes3 options SourceYeasen · Official

Cat. No. 96472ES25

现货 $1,135.00

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96472ES25 25 μg $1,135.00 现货 Enquire

96472ES60 100 μg $4,505.00 现货 Enquire

96472ES76 500 μg $15,005.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No96472ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 COVID-19研究用于SARS-CoV-2刺突蛋白及相关突变研究

适用物种种属 SARS-CoV-2

检测方法 ELISA SDS-PAGE HPLC LAL法 Bis-Tris PAGE

生物特性参数 蛋白类型重组蛋白 种属来源SARS-CoV-2 (Virus) 生物活性描述ELISA Data 1: Immobilized SARS-COV-2 Spike S1 (N501Y) His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2 hFc Tag with the EC50 of 66.2ng/ml determined by ELISA. 应用COVID-19研究

样本类型 重组蛋白

技术参数 表达系统HEK293 标签C-His 分子量The protein has a predicted MW of 76 kDa. Due to glycosylation the protein migrates to 115-140 kDa based on Bis-Tris PAGE result. 纯度> 95% as determined by SDS-PAGE > 90% as determined by HPLC 内毒素水平Less than 1 EU per μg by the LAL method. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 储存条件-25~-15℃保存，收到货之日起，有效期1年。复溶后，-85~-65℃保存，3个月有效期。避免反复冻融。 表达序列Val16-Arg685 AccessionYP_009724390.1 复溶方法用蒸馏水溶解冻干的蛋白。如果规格为25μg或者100μg，建议复溶到0.5mg/mL的浓度。对于500μg或1mg的冻干粉，建议以1mg/mL的浓度复溶。复溶过程中请不要剧烈涡旋或者剧烈摇晃。将复溶后的蛋白溶液按照需求进行分装，储存于至-80℃，避免反复冻融。外观Lyophilized Powder 活性EC50=66.2 ng/ml (ELISA测定与Human ACE2结合)