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Product data sheet PD-40209ES
LDH Cytotoxicity Assay Kit 乳酸脱氢酶(LDH)细胞毒性检测试剂盒 LDH Cytotoxicity Assay Kit 乳酸脱氢酶(LDH)细胞毒性检测试剂盒
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LDH Cytotoxicity Assay Kit 乳酸脱氢酶(LDH)细胞毒性检测试剂盒

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细胞毒性检测
Product no.40209ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 40209ES76 现货 $1,298.00 促$1,372.00
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Cat.No Spec Price Status
40209ES76 500 T $1,298.00促$1,372.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No40209ES
Specification500 T
Origin翌圣生物科技(上海)股份有限公司
应用方法 细胞毒性检测定量分析细胞毒性及细胞损伤
检测方法 比色法
样本类型 细胞培养液 细胞裂解液
技术参数 储存条件-25~-15℃,避光保存 保质期1年 产品形式即用型
实验类型 LDH释放检测通过检测释放的LDH活性评估细胞膜完整性

FAQ

Q:血清会影响检测结果吗?

A:由于血清含有乳酸脱氢酶,建议血清的使用浓度不要超过 1%,并最好使用热灭活血清。

Q:收取细胞上清液中释放的 LDH,在冰箱放置过夜,第二天检测可以吗?

A:收取细胞上清液中释放的 LDH,可以在冰箱放置过夜,第二天检测。但是为了减少实验误差,建议客户最好是现取现测。

Q: 乳酸脱氢酶细胞毒性检测试剂盒,对照的死亡率怎么算

A: 这个试剂盒一般是计算样品的死亡率, 不计算对照的死亡率呀,而且样品的死亡率也要用对照的来计算。
Q:使用600nm或大于600nm的波长进行双波长检测是为什么?
A:使用600nm或大于600nm的波长作为参考波长进行双波长检测,主要目的是校正非特异性吸收或光散射引起的干扰,从而提高检测结果的准确性。(非必须做)

Documents & Citations

Published citations

[1] Yu L, Liu X, Ye X, et al. Monoclonal Antibodies against Zika Virus NS1 Protein Confer Protection via Fcγ Receptor-Dependent and -Independent Pathways. mBio. 2021;12(1):e03179-20. Published 2021 Feb 9. doi:10.1128/mBio.03179-20(IF:7.867)

[2] Guo G, Zhou H, Wang Q, et al. Nano-layered magnesium fluoride reservoirs on biomaterial surfaces strengthen polymorphonuclear leukocyte resistance to bacterial pathogens. Nanoscale. 2017;9(2):875-892. doi:10.1039/c6nr07729c(IF:7.760)

[3] Liao L, Jiang C, Chen J, et al. Synthesis and biological evaluation of 1,2,4-triazole derivatives as potential neuroprotectant against ischemic brain injury. Eur J Med Chem. 2020;190:112114. doi:10.1016/j.ejmech.2020.112114(IF:5.573)

[4] Wang D, Yuan T, Liu J, et al. ATG16L2 inhibits NLRP3 inflammasome activation through promoting ATG5-12-16L1 complex assembly and autophagy [published online ahead of print, 2022 Apr 15]. Eur J Immunol. 2022;10.1002/eji.202149764. doi:10.1002/eji.202149764(IF:5.532)

[5] Dai B, Fan M, Huang X, et al. Shuanghua decoction exerts anticancer activity by activating NLRP3 inflammasome via ROS and inhibiting NF-κB signaling in hepatocellular carcinoma cells. Phytomedicine. 2022;103:154249. doi:10.1016/j.phymed.2022.154249(IF:5.340)

[6] Niu X, Lin L, Liu L, Yu Y, Wang H. Antifungal activity and molecular mechanisms of mulberrin derivatives against Colletotrichum gloeosporioides for mango storage [published online ahead of print, 2022 Jun 20]. Int J Food Microbiol. 2022;378:109817. doi:10.1016/j.ijfoodmicro.2022.109817(IF:5.277)

[7] Cai B, Liao C, He D, et al. Gasdermin E mediates photoreceptor damage by all-trans-retinal in the mouse retina. J Biol Chem. 2022;298(2):101553. doi:10.1016/j.jbc.2021.101553(IF:5.157)

[8] Lin CX, Gu JL, Cao JM. The acute toxic effects of platinum nanoparticles on ion channels, transmembrane potentials of cardiomyocytes in vitro and heart rhythm in vivo in mice. Int J Nanomedicine. 2019;14:5595-5609. Published 2019 Jul 22. doi:10.2147/IJN.S209135(IF:4.471)

[9] Hu T, Xu H, Wang C, Qin H, An Z. Magnesium enhances the chondrogenic differentiation of mesenchymal stem cells by inhibiting activated macrophage-induced inflammation. Sci Rep. 2018;8(1):3406. Published 2018 Feb 21. doi:10.1038/s41598-018-21783-2(IF:4.122)

[10] Yang Y, Wang X, Gao Y, Wang H, Niu X. Insight into the Dual inhibitory Mechanism of verbascoside targeting serine/threonine phosphatase Stp1 against Staphylococcus aureus. Eur J Pharm Sci. 2021;157:105628. doi:10.1016/j.ejps.2020.105628(IF:3.616)

[11] Wang F, Liu J, Wang D, Yao Y, Jiao X. Knockdown of circ_0007290 alleviates oxygen-glucose deprivation-induced neuronal injury by regulating miR-496/PDCD4 axis. Metab Brain Dis. 2022;37(3):807-818. doi:10.1007/s11011-021-00900-7(IF:3.584)

[12] Su Q, Wang J, Liu F, Zhang Y. Blocking Parkin/PINK1-mediated mitophagy sensitizes hepatocellular carcinoma cells to sanguinarine-induced mitochondrial apoptosis. Toxicol In Vitro. 2020;66:104840. doi:10.1016/j.tiv.2020.104840(IF:2.959)

[13] Lv H, Fang T, Kong F, et al. Dryocrassin ABBA ameliorates Streptococcus pneumoniae-induced infection in vitro through inhibiting Streptococcus pneumoniae growth and neutralizing pneumolysin activity. Microb Pathog. 2021;150:104683. doi:10.1016/j.micpath.2020.104683(IF:2.914)

[14] Liu XD, Li YG, Wang GY, et al. Metformin protects high glucose‑cultured cardiomyocytes from oxidative stress by promoting NDUFA13 expression and mitochondrial biogenesis via the AMPK signaling pathway. Mol Med Rep. 2020;22(6):5262-5270. doi:10.3892/mmr.2020.11599(IF:2.100)

[15] Wang T, Zhu L, Liu H, Yu G, Guo Y. Picroside II Protects SH-SY5Y Cells From Autophagy and Apoptosis Following Oxygen Glucose Deprivation/Reoxygen Injury by Inhibiting JNK Signal Pathway. Anat Rec (Hoboken). 2019;302(12):2245-2254. doi:10.1002/ar.24214(IF:1.329)

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