Permeabilization Wash Buffer 破膜剂

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流式细胞术流式细胞检测

Product no.40403ES Sizes1 options Documents1 files SourceYeasen · Official

Cat. No. 40403ES64 现货 ¥360.00

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40403ES64 150 mL ¥360.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No40403ES

Specification150 mL

Origin翌圣生物科技(上海)股份有限公司

技术平台 流式细胞术

应用方法 流式细胞检测检测细胞内抗原及DNA

检测方法 流式细胞检测

生物特性参数应用流式细胞检测

样本类型细胞

技术参数 存储温度2~8℃ 储存条件2~8℃避光保存，勿冰冻 有效期一年

实验类型 细胞内抗原检测用于细胞膜破膜处理以便抗体进入细胞

FAQ

Q:做核内因子的流式分析，这个破膜剂能破核膜吗？

A:不能，皂素是相对理想的细胞破膜剂，但无法破核膜，若需破核膜还是要用 Triton-X 和Tween-20。

Q:破膜剂能不能先染膜抗体，然后破膜后再染细胞里面的，还是说需要破膜之后一起染？

A:先固定，再破膜，再染色，需要破膜之后一起染。

Q: 这个破膜剂破膜是可逆的吗？用于免疫荧光中，使用该破膜剂后封闭液、抗体稀释液及洗涤液是否都可以使用这款破膜剂？

A: 是可逆的，该破膜剂为皂素。封闭液推荐使用该破膜剂配置1%的BSA，同样可以使用该破膜剂用于抗体稀释以及洗涤液。

Documents & Citations

细胞功能检测和探针整体方案 Open document

Published citations

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[3] Xiong C, Mao Y, Wu T, et al. Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in Pichia pastoris and Evaluation of Antitumor Activity In Vivo. Int J Mol Sci. 2018;19(10):2900. Published 2018 Sep 25. doi:10.3390/ijms19102900(IF:3.687)

[4] Ding D, Yang X, Luan HQ, Wu XT, Sun LW, Fan YB. The microgravity induces the ciliary shortening and an increased ratio of anterograde/retrograde intraflagellar transport of osteocytes. Biochem Biophys Res Commun. 2020;530(1):167-172. doi:10.1016/j.bbrc.2020.06.119(IF:3.575)

[5] Hao W, Shi S, Zhou S, Wang X, Nie S. Aspirin inhibits growth and enhances cardiomyocyte differentiation of bone marrow mesenchymal stem cells. Eur J Pharmacol. 2018;827:198-207. doi:10.1016/j.ejphar.2018.03.016(IF:3.040)

[6] Shi S, Wu X, Wang X, et al. Differentiation of Bone Marrow Mesenchymal Stem Cells to Cardiomyocyte-Like Cells Is Regulated by the Combined Low Dose Treatment of Transforming Growth Factor-β1 and 5-Azacytidine. Stem Cells Int. 2016;2016:3816256. doi:10.1155/2016/3816256(IF:2.813)

[7] Xue M, Liang H, Tang Q, et al. The Protective and Immunomodulatory Effects of Fucoidan Against 7,12-Dimethyl benz[a]anthracene-Induced Experimental Mammary Carcinogenesis Through the PD1/PDL1 Signaling Pathway in Rats. Nutr Cancer. 2017;69(8):1234-1244. doi:10.1080/01635581.2017.1362446(IF:2.447)