Q&A Community - BioReagent Hub

5ans

222views

For flow cytometry detection of mouse splenocytes, what blocking agent should be used with PE anti-rat secondary antibody to reduce nonspecific binding?

Compliance 何何研发 2026-08-29 #Flow cytometry #PE secondary antibody #Anti-rat

CD4 Elisa Kit

5ans

278views

Has anyone compared prices for switching the IHC secondary antibody from a universal type to an anti-rabbit/mouse dual-label one?

Supplier & Sourcing RReagentPlus3077 2026-09-01 #Immunohistochemistry #IHC secondary antibody #Double-labeled

5ans

183views

Immunofluorescence tissue sections require Alexa Fluor 594 anti-mouse secondary antibody; which supplier has the fastest availability from stock?

Medical Devices WWendy1455 2026-09-14 #Immunofluorescence #Alexa Fluor 594 #Donkey Anti-Mouse

5ans

211views

For Western blotting, when using an HRP-labeled goat anti-rabbit secondary antibody at a 1:5000 dilution, what should I do if the background is still high?

Supplier & Sourcing KKitHub1516 2026-09-15 #Western blot #HRP-conjugated secondary antibody #High background

5ans

210views

Immunofluorescence double staining requires primary antibodies from different species. How can cross-reactivity be assessed?

Lab Techniques 试试剂同学9920 2026-09-03 #Immunofluorescence #Primary antibody #Cross-reactivity

5ans

194views

Flow cytometry detection of CD molecules in human peripheral blood, direct-labeled primary antibody recommendations for clones and fluorophore combinations.

Compliance TTonyTube518 2026-09-03 #Flow cytometry #Directly labeled primary antibody #CD molecule

CD4 Elisa Kit

5ans

242views

For immunohistochemistry on paraffin sections, is it more cost-effective to use ready-to-use or concentrated primary antibodies?

Lab Techniques 飞飞飞大姐 2026-09-05 #Immunohistochemistry #Primary antibody #Paraffin section

5ans

238views

For WB detection of a phosphorylated protein in mouse liver tissue, how should the primary antibody be selected?

Lab Techniques KKevin5578 2026-09-17 #Western blot #Primary antibody #Mouse tissue

5ans

232views

How should the remaining wells of an ELISA kit microplate be stored after stripping the strips? Is the inter-batch variation large?

Compliance DDavid 2026-08-27 #ELISA kit #Microplate #Strip

ELISA试剂盒

FMDV-NSPABC-Ab Elisa...

5ans

329views

The sample volume of cell culture supernatant is very limited. What is the minimum volume in microliters that can be used with the ELISA kit?

Supplier & Sourcing 余余洋辉 2026-09-07 #ELISA kit #Cell supernatant #Small sample

ELISA试剂盒

IFN-β Elisa Kit

5ans

228views

For batch testing of rat TNF-α, how do the lead time and price of a 96T ELISA kit compare?

Storage & Shipping 杨杨娜2860 2026-09-12 #ELISA kit #TNF-α #Rat

ELISA试剂盒

FMDV-NSPABC-Ab Elisa...

5ans

344views

For human IL-6 ELISA kits used to measure serum samples, are there large differences in recovery rates among different brands?

Compliance VVivi3152 2026-08-31 #ELISA kit #IL-6 #Serum samples

ELISA试剂盒

IL-6 Elisa Kit

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