Q&A Community - BioReagent Hub

5ans

173views

How should adapter dimers be optimized when they are too high after微量cfDNA library preparation?

Medical Devices LLisa7850 2026-09-04 #cfDNA #Linker dimer #Micro-library construction

5ans

241views

Can group buying or tiered pricing be negotiated for bulk purchases of NGS library preparation kits?

Compliance JJackLab4776 2026-08-30 #Procurement price comparison #Tumor panel #Whole exome

5ans

214views

Can the fragmentation enzyme in the library preparation kit replace sonication shearing?

Medical Devices RReagent8049 2026-09-18 #Metagenomics #Enzymatic fragmentation #Library construction time

5ans

254views

Which company's NGS library preparation kit for low-input FFPE samples is more reliable?

Reagent Selection RReagentLine2140 2026-09-10 #RNA library construction #FFPE #Low input amount

5ans

235views

When performing qPCR on blood RNA, there is always gDNA contamination. Are there any kits with UNG for contamination prevention?

Storage & Shipping NNina9207 2026-09-17 #UNG contamination prevention #qPCR kit #Blood RNA

5ans

221views

When using the ABI StepOnePlus for mouse gene expression, how should I choose between ROX and dye-based methods?

Reagent Selection 董董飞飞 2026-09-18 #qPCR kit #ROX #ABI StepOnePlus

5ans

234views

Our lab wants to purchase qPCR master mix in bulk. How should we compare prices between domestic and imported products?

Medical Devices 刘刘超敏 2026-09-07 #SYBR Green #qPCR premix #Bulk purchase

5ans

330views

Low SYBR Green qPCR amplification efficiency: which kit should be used for low-copy templates?

Medical Devices KKevin 2026-09-10 #qPCR kit #Low-copy template #Amplification efficiency

4ans

206views

For culturing HepG2 and RAW264.7, should DMEM high glucose or 1640 be used? Is glutamine supplementation needed?

Lab Techniques AAndyProtein 2026-04-25 #Cell culture medium #DMEM #RPMI1640

4ans

236views

Which brand of serum-free medium for mesenchymal stem cells is everyone using? Please compare prices and lead times.

Reagent Selection 王王倩文 2026-07-14 #Cell culture medium #Mesenchymal stem cells #Serum-free

4ans

238views

When using a certain imported serum-free medium for Expi293 suspension culture, cell clumping always occurs. Seeking an alternative solution.

Storage & Shipping OOscarLab 2026-09-07 #Cell culture medium #Serum-free medium #Suspension culture

4ans

246views

How to choose Oligo dT resin for in vitro transcribed mRNA vaccine purification?

Lab Techniques 张张磊6040 2026-08-18 #mRNA vaccine #In vitro transcription #Purification resin

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