Q&A Community - BioReagent Hub

5ans

213views

For flow cytometry detection of mouse splenocytes, what blocking agent should be used with PE anti-rat secondary antibody to reduce nonspecific binding?

Compliance 何何研发 2026-08-29 #Flow cytometry #PE secondary antibody #Anti-rat

Recombinant Biotinyl...

5ans

267views

Has anyone compared prices for switching the IHC secondary antibody from a universal type to an anti-rabbit/mouse dual-label one?

Supplier & Sourcing RReagentPlus3077 2026-09-01 #Immunohistochemistry #IHC secondary antibody #Double-labeled

5ans

176views

Immunofluorescence tissue sections require Alexa Fluor 594 anti-mouse secondary antibody; which supplier has the fastest availability from stock?

Medical Devices WWendy1455 2026-09-14 #Immunofluorescence #Alexa Fluor 594 #Donkey Anti-Mouse

Recombinant Alexa Fl...

5ans

203views

For Western blotting, when using an HRP-labeled goat anti-rabbit secondary antibody at a 1:5000 dilution, what should I do if the background is still high?

Supplier & Sourcing KKitHub1516 2026-09-15 #Western blot #HRP-conjugated secondary antibody #High background

5ans

200views

Immunofluorescence double staining requires primary antibodies from different species. How can cross-reactivity be assessed?

Lab Techniques 试试剂同学9920 2026-09-03 #Immunofluorescence #Primary antibody #Cross-reactivity

Recombinant Alexa Fl...

5ans

182views

Flow cytometry detection of CD molecules in human peripheral blood, direct-labeled primary antibody recommendations for clones and fluorophore combinations.

Compliance TTonyTube518 2026-09-03 #Flow cytometry #Directly labeled primary antibody #CD molecule

Recombinant Biotinyl...

5ans

232views

For immunohistochemistry on paraffin sections, is it more cost-effective to use ready-to-use or concentrated primary antibodies?

Lab Techniques 飞飞飞大姐 2026-09-05 #Immunohistochemistry #Primary antibody #Paraffin section

5ans

229views

For WB detection of a phosphorylated protein in mouse liver tissue, how should the primary antibody be selected?

Lab Techniques KKevin5578 2026-09-17 #Western blot #Primary antibody #Mouse tissue

5ans

218views

How should the remaining wells of an ELISA kit microplate be stored after stripping the strips? Is the inter-batch variation large?

Compliance DDavid 2026-08-27 #ELISA kit #Microplate #Strip

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

315views

The sample volume of cell culture supernatant is very limited. What is the minimum volume in microliters that can be used with the ELISA kit?

Supplier & Sourcing 余余洋辉 2026-09-07 #ELISA kit #Cell supernatant #Small sample

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

212views

For batch testing of rat TNF-α, how do the lead time and price of a 96T ELISA kit compare?

Storage & Shipping 杨杨娜2860 2026-09-12 #ELISA kit #TNF-α #Rat

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

327views

For human IL-6 ELISA kits used to measure serum samples, are there large differences in recovery rates among different brands?

Compliance VVivi3152 2026-08-31 #ELISA kit #IL-6 #Serum samples

ELISA试剂盒

Plant PDH E1αElisa K...

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