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3ans

231views

Which supplier provides Western blot internal reference antibodies with fast delivery and small batch-to-batch variation?

Storage & Shipping 采采购老手 2026-01-27 #Antibody Procurement #Western blot #Loading control antibody

Plant PDH E1αElisa K...

3ans

265views

How to choose an ELISA kit for the detection of IL-6 and TNF-α in mouse serum?

Supplier & Sourcing 徐徐娟5472 2026-05-04 #ELISA kit #Inflammatory factors #Mouse serum

ELISA试剂盒

Plant PDH E1αElisa K...

4ans

246views

For extracting RNA from large batches of animal tissues, should Trizol be domestic or imported? How should delivery time and price be chosen?

Supplier & Sourcing TTony1002 2026-07-10 #Trizol #Domestic substitution #Price

4ans

237views

After collecting rat brain tissue from the animal room, should it be placed in liquid nitrogen first or soaked in Trizol first for preservation? Does this affect RNA integrity?

Reagent Selection TTube 2026-06-16 #Trizol #Sample preservation #Rat brain tissue

4ans

179views

The intestinal tissue collected from the chicken farm is very turbid when lysed with Trizol reagent, and the RNA yield is low. What should I do?

Storage & Shipping LLabing7973 2026-04-27 #Trizol #Intestinal tissue #RNA yield

4ans

248views

When using Trizol to extract RNA from animal liver, the 260/230 ratio is only 1.2. Is this due to fat interference?

Lab Techniques 董董静娟 2026-07-02 #RNA extraction #Trizol #Animal liver

4ans

200views

When performing animal liver protein purification, can PMSF and cocktail be added together?

Storage & Shipping 老老王君1105 2026-08-23 #Phenylmethylsulfonyl fluoride #Protease inhibitor cocktail #Protein purification

Recombinant Biotinyl...

4ans

220views

High background when using Triton X-100 for protein extraction from mouse brain tissue; seeking lysis conditions.

Reagent Selection 阿阿宝小助手2275 2026-05-24 #Triton X-100 #Western blot #Mouse hippocampus

5ans

234views

Rat serum alanine aminotransferase assay kit, is a sample volume of only 20 microliters sufficient?

Reagent Selection 李李艳燕2808 2026-09-02 #Biochemical reagent kit #ALT testing #Rat serum

5ans

177views

ELISA for measuring IL-1β and IL-10 in cell supernatants: how to choose when there are large differences among different brands?

Compliance 蒋蒋斌 2026-09-24 #ELISA #Cytokine detection #Batch-to-batch variation

Plant PDH E1αElisa K...

HMG-COA Elisa Kit

5ans

210views

Does serum-free medium for NK cells still require additional supplementation with IL-2 and serum replacement?

Compliance 张张助理 2026-08-28 #Serum-free medium #NK cells #IL-2

5ans

247views

Will 1% Triton X-100 affect the results when using a BCA kit to measure protein in cell lysates?

Reagent Selection PProteinLab5271 2026-09-24 #BCA protein quantification #Cell lysis buffer #Detergent compatibility

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