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#Primary cells

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4ans

257views

For primary cell transfection, which endotoxin-free plasmid extraction kit has the lowest residual?

Storage & Shipping AAndy2032 2026-04-01 #Plasmid extraction #Endotoxin removal #Primary cells

4ans

197views

Will adding penicillin-streptomycin to primary rat fibroblasts affect their growth?

Storage & Shipping RReagenting8982 2026-09-07 #Penicillin-Streptomycin #Primary cell culture #Cytotoxicity

4ans

201views

When performing primary hepatocyte counting, why is the viability always low after trypan blue staining?

Medical Devices 唐唐强阳 2026-09-21 #Trypan blue #Primary cells #Cell counting

4ans

222views

When primarily isolating mouse embryonic fibroblasts, how should the trypsin concentration and digestion time be determined?

Lab Techniques KKitLab 2026-06-19 #Trypsin #Primary cell isolation #MEF

4ans

235views

What should be added to DMEM when isolating lymphocytes from rat spleen for culture? What is the lead time?

Medical Devices LLily 2026-06-27 #DMEM medium #Primary cell culture #Rat spleen

4ans

244views

For avian disease diagnosis involving primary cell isolation, do serum batches have a significant impact?

Lab Techniques SScienceOne442 2026-03-11 #Fetal Bovine Serum #Primary cells #Poultry Disease Diagnosis

4ans

274views

How to optimize the concentration and time of EDTA free trypsin for primary neuron digestion?

Compliance PPCRPro1352 2026-08-03 #EDTA-free Trypsin #Primary cells #Cell dissociation

4ans

235views

Does FBS from Australia and South America have a significant impact on the differentiation of primary macrophages?

Compliance 胡胡文晨5245 2026-06-02 #Fetal Bovine Serum #Primary cells #Source comparison

0ans

245views

Does high endotoxin levels in recombinant proteins have significant side effects on primary cells? Please recommend low endotoxin batches

Compliance FFlask 2026-08-13 #Recombinant protein #Endotoxin #Primary cells

3ans

256views

What is the effective concentration of recombinant human IL-2 to stimulate T cell proliferation?

Lab Techniques TTony4198 2026-09-18 #Recombinant protein #Cytokine #Primary cell culture

3ans

264views

Is the appearance of flocculent sediment in thawed fetal bovine serum abnormal or normal?

Storage & Shipping 周周辉娟 2026-08-03 #Fetal Bovine Serum #Precipitation #Filtration

3ans

320views

What is the generally safe requirement for the endotoxin content in fetal bovine serum for cell culture?

Lab Techniques 冯冯大师9641 2026-07-13 #Fetal Bovine Serum #Endotoxin #Primary cell culture

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