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2ans

109views

医院样本库收呼吸道标本，病毒采样试剂盒保存液会抑制PCR吗

Storage & Shipping 杨杨细胞 2026-02-13 #Respiratory tract specimens #Virus Sampling Kit #Preservation solution

3ans

219views

Looking for recommendations for a protease inhibitor cocktail that is available locally in Xi'an with next-day delivery.

Compliance RReagent2565 2026-08-25 #Protease inhibitor #Xi'an Bioreagent #Protein purification

0ans

210views

Which is more suitable for tissue protein extraction: domestic protease inhibitor cocktail or imported aliquoted product?

Supplier & Sourcing NNinaProtein 2026-04-15 #Protease inhibitor #Protein extraction #Western blot

3ans

281views

Looking for recommendations for bioreagent companies with stable protease inhibitor cocktails.

Compliance 科科研小助手 2026-04-20 #Protein purification #Protease inhibitor #Bioreagent company

4ans

206views

When performing animal liver protein purification, can PMSF and cocktail be added together?

Storage & Shipping 老老王君1105 2026-08-23 #Phenylmethylsulfonyl fluoride #Protease inhibitor cocktail #Protein purification

Recombinant Biotinyl...

4ans

210views

When extracting protein from animal tissues, how long does PMSF remain effective after being added to the lysis buffer?

Lab Techniques PProtein2430 2026-04-18 #Phenylmethylsulfonyl fluoride #Protease inhibitor #Animal tissue

4ans

311views

Can the SDS used for culturing cells and raising animals be used interchangeably? For mycoplasma detection lysis.

Medical Devices EExtractPro9879 2026-03-04 #SDS #Mycoplasma testing #Animal samples

4ans

239views

When extracting proteins from animal tissues, if trypsin digestion is used beforehand, is it necessary to add inhibitors to prevent degradation?

Compliance RReagent1537 2026-08-01 #Trypsin #Protease inhibitor #Tissue digestion

5ans

229views

Will adding too much reverse transcriptase inhibit downstream qPCR? How many units are generally appropriate to add to a 20 µL system?

Storage & Shipping NNinaAssay36 2026-09-13 #Reverse transcriptase #RT-qPCR #System optimization

5ans

185views

When performing RNA reverse transcription from plant leaves, inhibition often occurs. Which reverse transcriptase has strong resistance to polysaccharides and polyphenols?

Reagent Selection BBufferLab6335 2026-09-16 #Reverse transcriptase #Plant RNA #Polysaccharides and polyphenols

5ans

233views

For extracting plasma cell-free DNA using the magnetic bead method, are there any kits recommended that can remove heparin inhibition?

Reagent Selection LLab9067 2026-09-15 #Free nucleic acid #Magnetic bead method #Plasma

3ans

169views

What should be done if the amplification curve of a molecular diagnostic kit rises late, and the negative control also shows a slight rise?

Lab Techniques RResearchOne2518 2026-09-12 #Molecular diagnostic kit #qPCR #Amplification curve

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