Q&A Community - BioReagent Hub

4ans

178views

Does DTT added to mouse embryo culture medium need to be filtered? What concentration is appropriate?

Lab Techniques LLeo3731 2026-03-01 #Embryo culture #DTT #Mouse

4ans

233views

What final concentration and incubation time should be used when treating mouse splenocytes with DTT for flow cytometry?

Supplier & Sourcing 李李文博 2026-03-26 #Flow cytometry #DTT #Mouse splenocytes

4ans

200views

DTT is used in both laboratory animal maintenance and electrophoresis. What size packaging is the most cost-effective to buy?

Medical Devices RReagent5972 2026-04-23 #DTT #Procurement #Lead time

4ans

174views

When extracting mouse liver protein, how much DTT should be added? The sample amount is about 100 mg.

Storage & Shipping 质质控小哥 2026-05-29 #Protein extraction #WB #DTT

4ans

313views

Can the SDS used for culturing cells and raising animals be used interchangeably? For mycoplasma detection lysis.

Medical Devices EExtractPro9879 2026-03-04 #SDS #Mycoplasma testing #Animal samples

4ans

227views

Can the cell lysis buffer prepared with SDS still be used after being stored at 4°C for one week? Measuring chicken peripheral blood lymphocytes.

Reagent Selection 蔡蔡燕7163 2026-08-10 #SDS #Red Blood Cell Lysis Buffer #Poultry

4ans

162views

For SDS purchased by the animal facility for use as electrophoresis buffer, is there a large purity difference between domestic and imported products?

Lab Techniques 曹曹文婷 2026-05-19 #SDS #Electrophoresis #Domestic reagents

4ans

211views

When SDS is used to lyse mouse liver tissue, foaming always occurs, affecting subsequent protein quantification. What should be done?

Reagent Selection 田田科研 2026-04-02 #SDS #Protein extraction #Mouse liver

4ans

181views

How many times should samples be diluted for the Coomassie Brilliant Blue method for goat milk whey protein detection?

Lab Techniques 郭郭鹏 2026-05-02 #Coomassie Brilliant Blue #Goat milk #Whey protein

4ans

191views

For quantifying bovine serum protein, should Coomassie Brilliant Blue G250 or BCA be used?

Compliance 袁袁小妹 2026-03-30 #Coomassie Brilliant Blue G250 #Bovine serum #BCA method

BCA Elisa Kit

4ans

267views

After extracting protein from porcine muscle tissue, what should I do if Coomassie Brilliant Blue staining always results in a dark background?

Storage & Shipping 谢谢娟佳 2026-07-28 #Coomassie Brilliant Blue #Muscle tissue #SDS-PAGE

Pro Elisa Kit

4ans

250views

Is it reliable to use Coomassie Brilliant Blue for measuring crude protein in chicken farm feed?

Medical Devices PProteinHub8367 2026-07-06 #Coomassie Brilliant Blue #Feed protein #Breeding farm

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