Q&A Community - BioReagent Hub

5ans

353views

After the recombinant cytokine lyophilized powder is dissolved, repeated freeze-thaw cycles cause a severe drop in activity. What should be done?

Storage & Shipping 林林小小 2026-08-28 #Recombinant cytokines #Lyophilized powder #Activity

5ans

214views

For intracellular flow cytometry staining of IL-17A and IFN-γ, must the stimulant and blocking agent be from the same brand?

Supplier & Sourcing BBuffer1209 2026-09-05 #Flow cytometry #Intracellular cytokines #Stimulant

IL-1β Elisa Kit

IFN-β Elisa Kit

5ans

190views

ELISA for measuring IL-1β and IL-10 in cell supernatants: how to choose when there are large differences among different brands?

Compliance 蒋蒋斌 2026-09-24 #ELISA #Cytokine detection #Batch-to-batch variation

IL-1β Elisa Kit

IL-10 Elisa Kit

5ans

200views

Which supplier offers recombinant mouse TNF-α and IL-6 with low endotoxin levels and fast availability from stock?

Compliance TTube404 2026-09-23 #Recombinant cytokines #Endotoxin #Macrophages

IL-6 Elisa Kit

Plant NF-κBElisa Kit

5ans

219views

Must the medium be switched to exosome-depleted fetal bovine serum for extracellular vesicle isolation?

Lab Techniques WWendy432 2026-09-10 #Fetal Bovine Serum #Exosomes #Ultracentrifugation

Pro Elisa Kit

5ans

255views

South American fetal bovine serum is experiencing price increases and shortages. Please recommend brands with stable lead times.

Compliance RResearchTech2274 2026-08-27 #Fetal Bovine Serum #Procurement price comparison #Lead time

5ans

277views

After thawing fetal bovine serum, if white flocculent precipitate appears, can it still be used?

Lab Techniques 孙孙鹏 2026-09-09 #Fetal Bovine Serum #Primary culture #Serum precipitate

5ans

231views

What should be done if hybridoma antibody yield fluctuates greatly after changing the fetal bovine serum lot?

Lab Techniques 赵赵静燕 2026-09-24 #Fetal Bovine Serum #Hybridoma #Antibody production

5ans

227views

Does serum-free medium for NK cells still require additional supplementation with IL-2 and serum replacement?

Compliance 张张助理 2026-08-28 #Serum-free medium #NK cells #IL-2

IL-2 Elisa Kit

5ans

223views

Does the direct switch of CHO cells from serum-containing to serum-free medium require an adaptation step?

Medical Devices LLisaLab 2026-09-01 #Serum-free medium #CHO cells #Domestication

5ans

244views

Which company's serum-free mesenchymal stem cell medium has small batch-to-batch variation and a reasonable price?

Storage & Shipping 周周燕燕 2026-09-09 #Serum-free medium #Mesenchymal stem cells #Batch-to-batch variation

5ans

280views

Will the antibody expression level of 293 cells drop after replacing serum with serum-free medium?

Storage & Shipping BBuffer9515 2026-09-11 #Serum-free medium #HEK293 #Antibody expression

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