Q&A Community - BioReagent Hub

3ans

233views

For HBV DNA quantitative detection molecular diagnostic kits, is it more reliable to choose domestic or imported ones?

Lab Techniques WWangExtract3988 2026-09-01 #Molecular diagnostic kit #HBV DNA #Fluorescence quantitative PCR

3ans

267views

Does a certain brand's multiplex molecular diagnostic kit for respiratory pathogens recently have stock available? What is the price?

Storage & Shipping 马马抗体2344 2026-09-11 #Molecular diagnostic kit #Respiratory pathogens #Lead time

3ans

247views

Will repeated freeze-thaw cycles after dissolving primer and probe dry powder affect the experimental results?

Lab Techniques 细细胞君 2026-08-26 #Primer storage #Probe stability #Freeze-thaw

3ans

286views

In multiplex qPCR, will the fluorescent groups of different probes interfere with each other?

Lab Techniques MMediaer111 2026-09-06 #Multiplex qPCR #Fluorescent labeling #Primer and probe

3ans

231views

For bulk customized primers and probes, which company offers better lead times and pricing?

Supplier & Sourcing LLabBase2006 2026-09-18 #Primer synthesis #Probe customization #Price comparison

3ans

178views

How should parameters be adjusted for nonspecific amplification of qPCR primers and probes?

Reagent Selection 董董耗材 2026-09-13 #qPCR #Primer design #Probe optimization

3ans

184views

For library adapter ligation, is T4 ligase or rapid ligase less prone to forming dimers?

Storage & Shipping WWendyResearch189... 2026-09-23 #Tool enzymes #T4 ligase #Library construction

3ans

285views

For qPCR with low-input RNA, which reverse transcriptase brand is more reliable?

Reagent Selection 彭彭静斌8274 2026-08-28 #Tool enzymes #Reverse transcriptase #Low-input RNA

3ans

187views

For point mutations in a 3 kb fragment, is Q5 or KOD One high-fidelity polymerase better?

Lab Techniques EEmmaProtein8173 2026-08-28 #Tool enzymes #High-fidelity polymerase #Point mutation

3ans

210views

Can domestic restriction endonucleases be used for double digestion as a substitute for NEB's?

Lab Techniques 飞飞飞小哥8329 2026-09-06 #Tool enzymes #Restriction endonuclease #Double enzyme digestion

3ans

233views

What are the approximate minimum order quantity and lead time for custom recombinant proteins?

Reagent Selection 曾曾采购 2026-09-18 #Recombinant protein #Customization Services #Lead time

3ans

256views

The endotoxin level of His-tagged recombinant protein is relatively high. How can it be removed in the laboratory?

Supplier & Sourcing 唐唐采购8407 2026-08-27 #Recombinant protein #His tag #Endotoxin

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