Q&A Community - BioReagent Hub

4ans

184views

Does paraformaldehyde fixation absolutely have to be performed in a fume hood? The animal facility has limited conditions.

Lab Techniques 林林学生2023 2026-03-11 #Paraformaldehyde #Safety protection #Neonatal rat

4ans

266views

For batch fixation of tissues in the animal facility, is it more cost-effective to buy ready-made paraformaldehyde fixative or prepare it in-house?

Compliance 曹曹阳1693 2026-06-14 #Paraformaldehyde fixative solution #Zebrafish #Rats and mice

4ans

180views

After paraformaldehyde fixation, the cell nuclei appear blurred—is this a reagent batch issue?

Storage & Shipping DDavid 2026-07-22 #Paraformaldehyde #Immunohistochemistry #Rat liver

4ans

156views

How should paraformaldehyde be prepared for perfusion fixation of mouse brain tissue so that it does not become cloudy?

Compliance OOscar5927 2026-08-03 #Paraformaldehyde #Mouse perfusion #Tissue fixation

4ans

225views

Can the same batch of PBS used for culturing cells and raising animals in the laboratory be shared?

Compliance RResearchHub8536 2026-09-15 #Phosphate buffer solution #Cell culture #Animal experiments

4ans

211views

Can self-prepared PBS, aliquoted and sterilized, be used for rabbit perfusion fixation?

Storage & Shipping TTubeZone3890 2026-08-08 #Phosphate buffer solution #Animal perfusion #High-pressure steam sterilization

4ans

225views

Does diluting the vaccine with PBS for chicken injection affect the titer due to pH and osmolality deviations?

Lab Techniques 大大师小哥1267 2026-09-05 #Phosphate buffer solution #Vaccine Diluent #Aquaculture

4ans

226views

Does the PBS used for washing tissues in the animal room have to be purchased as sterile and calcium- and magnesium-free?

Lab Techniques RRex7790 2026-05-07 #Phosphate buffer solution #Animal experiments #Tissue sampling

4ans

181views

How much activity is lost when bulk trypsin is aliquoted and subjected to repeated freeze-thaw cycles?

Lab Techniques 老老王小陈5167 2026-03-09 #Trypsin #Aliquot and store #Repeated freeze-thaw

4ans

237views

When extracting proteins from animal tissues, if trypsin digestion is used beforehand, is it necessary to add inhibitors to prevent degradation?

Compliance RReagent1537 2026-08-01 #Trypsin #Protease inhibitor #Tissue digestion

4ans

220views

Imported trypsin has unstable lead times, and I want to find a domestic alternative for cell passaging. Does anyone have any recommendations?

Compliance 科科研控 2026-08-02 #Trypsin #Domestic substitution #Cell passaging

4ans

220views

When primarily isolating mouse embryonic fibroblasts, how should the trypsin concentration and digestion time be determined?

Lab Techniques KKitLab 2026-06-19 #Trypsin #Primary cell isolation #MEF

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