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5ans

272views

Consultation on antibody color configuration and usage for flow cytometry CD3/CD4/CD8 in mouse splenocytes

Medical Devices 彭彭明燕 2026-09-04 #Flow cytometry antibodies #Fluorescence color scheme #Mouse splenocytes

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5ans

215views

For flow cytometry detection of mouse splenocytes, what blocking agent should be used with PE anti-rat secondary antibody to reduce nonspecific binding?

Compliance 何何研发 2026-08-29 #Flow cytometry #PE secondary antibody #Anti-rat

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5ans

187views

Flow cytometry detection of CD molecules in human peripheral blood, direct-labeled primary antibody recommendations for clones and fluorophore combinations.

Compliance TTonyTube518 2026-09-03 #Flow cytometry #Directly labeled primary antibody #CD molecule

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4ans

199views

How can the antibody panel design and amounts be optimized for flow cytometric detection of CTLA-4 and LAG-3 in human PBMCs?

Reagent Selection MMark 2026-05-13 #Immune checkpoint #Flow cytometry antibodies #CTLA-4

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4ans

257views

To detect the CAR-positive rate of CD19 CAR-T cells, should Protein L or an anti-idiotypic antibody be used?

Compliance JJack1145 2026-09-03 #CAR-T cell therapy #Flow cytometry detection #Protein L

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4ans

206views

Seeking advice on antibody panel design for IL-17A and Foxp3 in Th17/Treg flow cytometry using mouse spleen single cells.

Compliance 陈陈小妹4696 2026-05-07 #Flow cytometry #Th17 #Treg

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4ans

194views

How should the dosage of anti-human CD3 monoclonal antibody for flow cytometric detection of human T cell surface be titrated?

Reagent Selection AAntibodyZone1727 2026-05-03 #Monoclonal antibody #Flow cytometry #CD3

5ans

194views

The mouse serum sample volume is limited, and I want to measure multiple cytokines in one run. Should I choose CBA or Luminex?

Medical Devices 研研发达人2657 2026-09-08 #Multiplex assay #Cytokine #Flow cytometry

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3ans

287views

When purchasing flow cytometry antibodies, is it necessary for the isotype control to be from the same brand and have the same fluorescence as the primary antibody?

Reagent Selection LLisa 2026-09-04 #Flow cytometry antibodies #Isotype control #Regulatory T cells

3ans

248views

After fixation and permeabilization, which flow cytometry antibodies can still be used to stain intracellular markers? Please recommend compatible fluorochromes.

Supplier & Sourcing AAmy9320 2026-09-22 #Intracellular flow cytometry #Fixation and permeabilization #IL-17A

3ans

192views

What are the appropriate cell and antibody amounts for flow cytometry antibody titration?

Storage & Shipping DDavid5521 2026-09-04 #Flow cytometry antibodies #Antibody titration #PBMC

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3ans

232views

For multicolor flow cytometry of mouse splenocytes, how should CD3/CD4/CD8 antibodies be paired with fluorophores to avoid interference?

Storage & Shipping RReagentLine8420 2026-09-07 #Flow cytometry antibodies #Multi-color color scheme #Mouse lymphoma

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