Tag

#Purification

All questions >

4ans

208views

When performing animal liver protein purification, can PMSF and cocktail be added together?

Storage & Shipping 老老王君1105 2026-08-23 #Phenylmethylsulfonyl fluoride #Protease inhibitor cocktail #Protein purification

Recombinant Biotinyl...

4ans

234views

How much does DMSO residue in monoclonal antibody purification samples affect mouse tail vein injection?

Supplier & Sourcing BBuffering7757 2026-06-29 #Dimethyl sulfoxide #Monoclonal antibody purification #Mouse injection

5ans

254views

After purification, the His-tagged protein is quantified using BCA. At what imidazole concentration will it interfere?

Medical Devices 许许艳燕6255 2026-09-09 #His-tagged protein #Imidazole interference #BCA quantification

Recombinant Biotinyl...

5ans

190views

After purifying a His-tagged recombinant protein, if you want to cleave off the tag, is TEV or thrombin more cost-effective?

Compliance RReagentHub3082 2026-09-04 #Recombinant protein purification #His tag #Protease cleavage

Recombinant Biotinyl...

5ans

246views

Which company's NGS library preparation kit for low-input FFPE samples is more reliable?

Reagent Selection RReagentLine2140 2026-09-10 #RNA library construction #FFPE #Low input amount

4ans

238views

How to choose Oligo dT resin for in vitro transcribed mRNA vaccine purification?

Lab Techniques 张张磊6040 2026-08-18 #mRNA vaccine #In vitro transcription #Purification resin

4ans

197views

After purification of His-tagged recombinant protein using a nickel column, the bands are impure. How can I optimize the conditions?

Compliance AAndy2058 2026-08-07 #Recombinant protein #His tag #Nickel column purification

Recombinant Biotinyl...

3ans

171views

Can microfluidic chips be used for plasma exosome isolation?

Medical Devices CCellTech4667 2026-09-12 #Exosomes #Microfluidic chip #Separation and purification

0ans

256views

Beekman dilute hydrochloric acid is cheaper but has too long a lead time. Is there an alternative?

Compliance RResearchOne3731 2026-06-06 #Beikeman Biology #Dilute hydrochloric acid #Lead time

4ans

215views

Is there a significant difference in the induction effect of domestic and imported IPTG inducers in BL21 (DE3)?

Reagent Selection EEmma8067 2026-06-05 #IPTG #Prokaryotic expression #Protein purification

Recombinant Biotinyl...

4ans

237views

What is the most suitable molar ratio for using HRP labeled streptavidin for ELISA?

Storage & Shipping SSampleing 2026-07-18 #HRP-labeled #Streptavidin #Molar ratio

Plant PDH E1αElisa K...

HMG-COA Elisa Kit

4ans

206views

What level of purity is required for dNTPs used in cloning sequencing?

Lab Techniques SSample 2026-08-20 #dNTPs #HPLC purification #Cloning

Community Rules

1SearchAvoid duplicates

2Be SpecificContext + tries + errors

3Answer WellGive steps and evidence

4AcceptBest answer first

Incentives

✓Points for accepted answers

✓Top answerers listed

✓Vendor support on board

✓Auto-linked products

Community Q&AQ&A CENTER

#Purification

Community Rules

Incentives