Tag

#qPCR

All questions >

4ans

224views

For quantitative cell gene expression analysis, the SYBR Green Ct values are too high, and I want to switch reagents. Any recommendations?

Supplier & Sourcing 马马燕 2026-05-25 #qPCR reagent #Gene expression #SYBR Green

2ans

181views

Will the performance of lyophilized reagents for pathogen nucleic acid detection decline after reconstitution? Are there any stabilizing formulation recommendations?

Compliance FFlaskLine 2026-09-16 #Lyophilized reagent #qPCR #Pathogen nucleic acid detection

3ans

167views

What should be done if the amplification curve of a molecular diagnostic kit rises late, and the negative control also shows a slight rise?

Lab Techniques RResearchOne2518 2026-09-12 #Molecular diagnostic kit #qPCR #Amplification curve

3ans

271views

In multiplex qPCR, will the fluorescent groups of different probes interfere with each other?

Lab Techniques MMediaer111 2026-09-06 #Multiplex qPCR #Fluorescent labeling #Primer and probe

3ans

162views

How should parameters be adjusted for nonspecific amplification of qPCR primers and probes?

Reagent Selection 董董耗材 2026-09-13 #qPCR #Primer design #Probe optimization

3ans

274views

For qPCR with low-input RNA, which reverse transcriptase brand is more reliable?

Reagent Selection 彭彭静斌8274 2026-08-28 #Tool enzymes #Reverse transcriptase #Low-input RNA

Recombinant Biotinyl...

4ans

196views

How to cut RNA from paraffin-embedded human tissue wax blocks for qPCR to reduce degradation?

Compliance VViviAssay5026 2025-11-15 #Human tissue wax block #RNA extraction #qPCR

4ans

253views

Does the presence or absence of RNase H activity have a significant impact on subsequent qPCR?

Compliance 老老王同学 2026-08-08 #Reverse transcriptase #RNase H #AMV

4ans

337views

How to choose reverse transcriptase for RT qPCR of low copy genes?

Supplier & Sourcing WWangResearch7772 2026-04-14 #Reverse transcriptase #RT-qPCR #Low copy

4ans

258views

How long does it usually take for a 5ml package of SYBR Green dye to arrive for qPCR?

Compliance EElisaMax 2026-07-14 #qPCR #SYBR Green I #Lead time

4ans

353views

How to prepare dNTPs for qPCR to switch to dUTP anti pollution system?

Supplier & Sourcing FFlask8008 2026-08-26 #qPCR #dUTP #dNTPs

4ans

187views

Which domestic manufacturers rank high for Taq enzyme and qPCR mix used in molecular experiments?

Supplier & Sourcing RRex5107 2026-08-27 #qPCR #Molecular biology reagents #Domestic substitution

Community Rules

1SearchAvoid duplicates

2Be SpecificContext + tries + errors

3Answer WellGive steps and evidence

4AcceptBest answer first

Incentives

✓Points for accepted answers

✓Top answerers listed

✓Vendor support on board

✓Auto-linked products

Community Q&AQ&A CENTER

#qPCR

Community Rules

Incentives