Q&A Community - BioReagent Hub

5ans

203views

When using BCA to quantify serum samples, low concentrations are always inaccurate. Are there any optimized conditions?

Reagent Selection JJack5561 2026-09-06 #Serum samples #Low-concentration protein #BCA quantification

5ans

251views

After purification, the His-tagged protein is quantified using BCA. At what imidazole concentration will it interfere?

Medical Devices 许许艳燕6255 2026-09-09 #His-tagged protein #Imidazole interference #BCA quantification

Recombinant Biotinyl...

5ans

214views

Our lab would like to purchase BCA kits in bulk. What is the availability and pricing for the 500-assay pack?

Storage & Shipping 张张佳倩 2026-09-04 #BCA Assay Kit #Bulk purchase #Price

5ans

247views

Will 1% Triton X-100 affect the results when using a BCA kit to measure protein in cell lysates?

Reagent Selection PProteinLab5271 2026-09-24 #BCA protein quantification #Cell lysis buffer #Detergent compatibility

5ans

240views

If recombinant protein is used to immunize mice for polyclonal antibody production and only 500 μg of protein is available, how should the adjuvant and immunization doses be arranged?

Compliance 徐徐婷 2026-09-13 #Recombinant protein #Antibody preparation #Immune adjuvant

5ans

220views

When buying recombinant human TNF-α protein, domestic and imported products differ quite a bit in price and lead time. How should I choose for cell experiments?

Reagent Selection LLily5054 2026-09-05 #Recombinant protein #Cytokine #Procurement price comparison

Plant NF-κBElisa Kit

5ans

283views

Most recombinant proteins expressed in E. coli are in inclusion bodies. Are there any mild refolding protocols?

Compliance RResearch9709 2026-09-19 #Recombinant protein #Inclusion bodies #Refolding

5ans

189views

After purifying a His-tagged recombinant protein, if you want to cleave off the tag, is TEV or thrombin more cost-effective?

Compliance RReagentHub3082 2026-09-04 #Recombinant protein purification #His tag #Protease cleavage

Recombinant Biotinyl...

5ans

221views

How should a titration experiment be performed after newly purchasing anti-human CD19-PE flow cytometry antibody?

Medical Devices 刘刘小小 2026-09-17 #Flow cytometry antibodies #Titration experiment #CD19

5ans

281views

When purchasing flow cytometry antibodies, is there a big difference in practice between different brands with the same clone number?

Compliance RResearchZone 2026-09-09 #Flow cytometry antibodies #Brand comparison #CD25

5ans

213views

Can flow cytometry antibodies for surface staining be used for intracellular staining after fixation and permeabilization?

Compliance DDavidScience1321 2026-08-30 #Flow cytometry antibodies #Intracellular staining #Th17

Recombinant Biotinyl...

5ans

267views

Consultation on antibody color configuration and usage for flow cytometry CD3/CD4/CD8 in mouse splenocytes

Medical Devices 彭彭明燕 2026-09-04 #Flow cytometry antibodies #Fluorescence color scheme #Mouse splenocytes

Recombinant Biotinyl...

Community Rules

1SearchAvoid duplicates

2Be SpecificContext + tries + errors

3Answer WellGive steps and evidence

4AcceptBest answer first

Incentives

✓Points for accepted answers

✓Top answerers listed

✓Vendor support on board

✓Auto-linked products

Community Q&AQ&A CENTER

Community Rules

Incentives