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4ans

191views

After purification of His-tagged recombinant protein using a nickel column, the bands are impure. How can I optimize the conditions?

Compliance AAndy2058 2026-08-07 #Recombinant protein #His tag #Nickel column purification

Recombinant Biotinyl...

5ans

193views

Transfection reagent for AAV packaging: How can the transfection efficiency of the three-plasmid system in HEK293T be improved?

Medical Devices KKevinBuffer4446 2026-09-16 #AAV packaging #HEK293T #Three-plasmid

5ans

249views

Which brand's culture medium offers the best cost-effectiveness for large-scale culture of suspension 293F cells?

Medical Devices EExtractLine6466 2026-09-12 #Suspension culture #HEK293F #AAV

3ans

287views

Can a fully automated nucleic acid extraction instrument use non-original deep-well plates and sealing films to run this molecular diagnostic kit?

Medical Devices RResearch 2026-09-12 #Molecular diagnostic kit #Fully automated extraction instrument #Companion consumables

3ans

218views

For HBV DNA quantitative detection molecular diagnostic kits, is it more reliable to choose domestic or imported ones?

Lab Techniques WWangExtract3988 2026-09-01 #Molecular diagnostic kit #HBV DNA #Fluorescence quantitative PCR

3ans

247views

The endotoxin level of His-tagged recombinant protein is relatively high. How can it be removed in the laboratory?

Supplier & Sourcing 唐唐采购8407 2026-08-27 #Recombinant protein #His tag #Endotoxin

Plant NF-κBElisa Kit

Recombinant Biotinyl...

3ans

198views

Is there a big difference in sensitivity between domestic and imported FISH hybridization kits, given that the price differs by a factor of two?

Lab Techniques DDavid 2026-09-12 #FISH #HER2 #Domestic vs. imported comparison

3ans

225views

Can the denaturation solution and neutralization solution in a Southern blotting kit be used interchangeably with other brands?

Reagent Selection LLeo8764 2026-09-12 #Southern blotting #Nucleic acid transfer #Reagent compatibility

3ans

242views

For paraffin section FISH, please recommend a digoxigenin-labeled hybridization kit with low background.

Supplier & Sourcing CCindyMedia9676 2026-08-29 #FISH #Paraffin section #Digoxigenin labeling

4ans

232views

Do paraffin-embedded tissue microarray sections from different manufacturers easily detach after staining?

Lab Techniques 马马敏5411 2026-05-05 #Paraffin-embedded tissue microarray #Detachment #Immunohistochemistry

4ans

232views

For IHC detection of F4/80 in mouse liver tissue, which primary antibody is less prone to nonspecific staining?

Compliance 杜杜涛娜4788 2026-01-24 #IHC #Primary antibody #F4/80

4ans

217views

Are tumor tissue microarrays stable for FISH probe validation?

Storage & Shipping 试试剂同学 2026-08-24 #Tumor tissue microarray #FISH #Probe validation

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