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4ans

140views

After grinding tumor tissue blocks, single cells stained with Annexin V/PI show many fragments. How should gating be performed?

Reagent Selection RResearch 2026-04-02 #Tumor tissue #Single-cell suspension #Flow cytometry gating

4ans

182views

For apoptosis experiments using primary rat hepatocytes, will trypsin digestion affect Annexin V staining?

Medical Devices 潘潘宇琳4195 2026-09-08 #Primary hepatocytes #Trypsin digestion #Annexin V

4ans

245views

Does the apoptosis kit require separate compensation adjustment when used with mouse splenocytes?

Storage & Shipping AAntibodyer2477 2026-06-29 #Mouse splenocytes #Flow cytometry compensation #Annexin V-FITC

4ans

241views

For extracting RNA from large batches of animal tissues, should Trizol be domestic or imported? How should delivery time and price be chosen?

Supplier & Sourcing TTony1002 2026-07-10 #Trizol #Domestic substitution #Price

4ans

236views

After collecting rat brain tissue from the animal room, should it be placed in liquid nitrogen first or soaked in Trizol first for preservation? Does this affect RNA integrity?

Reagent Selection TTube 2026-06-16 #Trizol #Sample preservation #Rat brain tissue

4ans

176views

The intestinal tissue collected from the chicken farm is very turbid when lysed with Trizol reagent, and the RNA yield is low. What should I do?

Storage & Shipping LLabing7973 2026-04-27 #Trizol #Intestinal tissue #RNA yield

4ans

246views

When using Trizol to extract RNA from animal liver, the 260/230 ratio is only 1.2. Is this due to fat interference?

Lab Techniques 董董静娟 2026-07-02 #RNA extraction #Trizol #Animal liver

4ans

192views

When performing animal liver protein purification, can PMSF and cocktail be added together?

Storage & Shipping 老老王君1105 2026-08-23 #Phenylmethylsulfonyl fluoride #Protease inhibitor cocktail #Protein purification

Recombinant Biotinyl...

5ans

216views

What transfection reagent yields high silencing efficiency for siRNA transfection in HepG2 cells?

Compliance WWang9305 2026-09-03 #siRNA #Transfection reagent #HepG2

5ans

173views

ELISA for measuring IL-1β and IL-10 in cell supernatants: how to choose when there are large differences among different brands?

Compliance 蒋蒋斌 2026-09-24 #ELISA #Cytokine detection #Batch-to-batch variation

Plant PDH E1αElisa K...

HMG-COA Elisa Kit

5ans

204views

Does serum-free medium for NK cells still require additional supplementation with IL-2 and serum replacement?

Compliance 张张助理 2026-08-28 #Serum-free medium #NK cells #IL-2

5ans

242views

After purification, the His-tagged protein is quantified using BCA. At what imidazole concentration will it interfere?

Medical Devices 许许艳燕6255 2026-09-09 #His-tagged protein #Imidazole interference #BCA quantification

Recombinant Biotinyl...

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